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Ribosomes are highly conserved large ribonucleoprotein (RNP) particles, consisting in yeast of a large 60S subunit and a small 40S subunit, that perform protein synthesis. Yeast ribosomes contain one copy each of four ribosomal RNAs (5S, 5.8S, 18S, and 25S; produced in two separate transcripts encoded within the rDNA repeat present as hundreds of copies on Chromosome 12) and 79 different ribosomal proteins (r-proteins), which are encoded by 137 different genes scattered about the genome, 59 of which are duplicated (7, 5). The 60S subunit contains 46 proteins and three RNA molecules: 25S RNA of 3392 nt, hydrogen bonded to the 5.8S RNA of 158 nt and associated with the 5S RNA of 121 nt. The 40S subunit has a single 18S RNA of 1798 nt and 33 proteins (8, 5). All yeast ribosomal proteins have a mammalian homolog (9).

In a rapidly growing yeast cell, 60% of total transcription is devoted to ribosomal RNA, and 50% of RNA polymerase II transcription and 90% of mRNA splicing are devoted to the production of mRNAs for r-proteins. Coordinate regulation of the rRNA genes and 137 r-protein genes is affected by nutritional cues and a number of signal transduction pathways that can abruptly induce or silence the ribosomal genes, whose transcripts have naturally short lifetimes, leading to major implications for the expression of other genes as well (10, 11, 12). The expression of some r-protein genes is influenced by Abf1p (13), and most are directly induced by binding of Rap1p to their promoters, which excludes nucleosomes and recruits Fhl1p and Ifh1p to drive transcription (14).

Ribosome assembly is a complex process, with different steps occurring in different parts of the cell. Ribosomal protein genes are transcribed in the nucleus, and the mRNA is transported to the cytoplasm for translation. The newly synthesized r-proteins then enter the nucleus and associate in the nucleolus with the two rRNA transcripts, one of which is methylated and pseudouridylated (view sites of modifications), and then cleaved into three individual rRNAs (18S, 5.8S, and 25S) as part of the assembly process (7). Separate ribosomal subunits are then transported from the nucleolus to the cytoplasm where they assemble into mature ribosomes before functioning in translation (15, 16). Blockage of subunit assembly, such as due to inhibition of rRNA synthesis or processing, results in degradation of newly synthesized r-proteins (17, 16). (For more information on the early steps of rRNA processing and small ribosomal subunit assembly, see the summary paragraph for the U3 snoRNA, encoded by snR17A and snR17B.)", "date_edited": "2007-02-14"}, "literature_overview": {"primary_count": 25, "additional_count": 51, "review_count": 7, "go_count": 3, "phenotype_count": 1, "disease_count": 0, "interaction_count": 68, "regulation_count": 9, "ptm_count": 12, "funComplement_count": 0, "htp_count": 29, "total_count": 185}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [], "URS_ID": null, "main_strain": "S288C", "regulation_overview": {"regulator_count": 14, "target_count": 0}, "reference_mapping": {"612181": 1, "592544": 2, "548623": 3, "526423": 4, "397935": 5, "2065584": 6, "592595": 7, "517471": 8, "556638": 9, "631529": 10, "554134": 11, "585697": 12, "608664": 13, "518051": 14, "592541": 15, "508692": 16, "508689": 17, "316136": 18}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: RPL24B", "date_created": "2000-05-19", "references": [{"id": 551590, "display_name": "SGD (2007)", "citation": "SGD (2007) Information without a citation in SGD ", "pubmed_id": null, "link": "/reference/S000073348", "year": 2007, "urls": []}]}, {"category": "Name", "history_type": "LSP", "note": "Name: L24e", "date_created": "2012-11-02", "references": [{"id": 397935, "display_name": "Jenner L, et al. (2012)", "citation": "Jenner L, et al. (2012) Crystal structure of the 80S yeast ribosome. Curr Opin Struct Biol 22(6):759-67", "pubmed_id": 22884264, "link": "/reference/S000150523", "year": 2012, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1016/j.sbi.2012.07.013"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/22884264"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: eL24", "date_created": "2016-08-09", "references": [{"id": 316136, "display_name": "Ban N, et al. (2014)", "citation": "Ban N, et al. (2014) A new system for naming ribosomal proteins. Curr Opin Struct Biol 24:165-9", "pubmed_id": 24524803, "link": "/reference/S000182084", "year": 2014, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1016/j.sbi.2014.01.002"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358319/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/24524803"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: rp29", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: L24B", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: L30B", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: YL21", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: RPL30B", "date_created": "2010-02-16", "references": []}, {"category": "Annotation change", "history_type": "SEQUENCE", "note": "Annotation change: RPL24B/YGR148C mRNA contains an intron in the 5' untranslated region (UTR).", "date_created": "2007-04-04", "references": [{"id": 508230, "display_name": "Juneau K, et al. (2007)", "citation": "Juneau K, et al. (2007) High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing. Proc Natl Acad Sci U S A 104(5):1522-7", "pubmed_id": 17244705, "link": "/reference/S000120506", "year": 2007, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1073/pnas.0610354104"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1780280/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/17244705"}]}, {"id": 510418, "display_name": "Miura F, et al. (2006)", "citation": "Miura F, et al. (2006) A large-scale full-length cDNA analysis to explore the budding yeast transcriptome. Proc Natl Acad Sci U S A 103(47):17846-51", "pubmed_id": 17101987, "link": "/reference/S000119659", "year": 2006, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1073/pnas.0605645103"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1693835/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/17101987"}, {"display_name": "Reference supplement", "link": "http://www.pnas.org/cgi/content/full/0605645103/DC1"}, {"display_name": "Reference supplement", "link": "http://yeast.utgenome.org/"}]}, {"id": 536678, "display_name": "Mitra G and Warner JR (1984)", "citation": "Mitra G and Warner JR (1984) A yeast ribosomal protein gene whose intron is in the 5' leader. J Biol Chem 259(14):9218-24", "pubmed_id": 6086628, "link": "/reference/S000079790", "year": 1984, "urls": [{"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/6086628"}]}, {"id": 549853, "display_name": "Cliften P, et al. (2003)", "citation": "Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6", "pubmed_id": 12775844, "link": "/reference/S000073948", "year": 2003, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1126/science.1084337"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/12775844"}, {"display_name": "Reference supplement", "link": "http://www.sciencemag.org/cgi/content/full/1084337/DC1"}]}, {"id": 612181, "display_name": "Baronas-Lowell DM and Warner JR (1990)", "citation": "Baronas-Lowell DM and Warner JR (1990) Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae. Mol Cell Biol 10(10):5235-43", "pubmed_id": 2204809, "link": "/reference/S000051769", "year": 1990, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1128/mcb.10.10.5235-5243.1990"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC361207/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2204809"}]}]}], "complexes": []} RPL24B Interactions | SGD

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Interaction Help

RPL24B / YGR148C Interactions

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference. Experimental details, phenotype, modification, and multiple mutant information are included when available.

Summary
The rpl24b null mutant is viable; the null mutant of paralog rpl24a is viable; the rpl24b rpl24a double mutant displays a synthetic growth defect.

Source: All physical and genetic interaction annotations listed in SGD are curated by BioGRID.

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Genetic Interactions

An interaction is defined as an experimentally observed physical or genetic interaction between two genes. There may be more than one row listed for the same interactor if the interaction between it and the given gene was observed in separate studies. All interactions listed in SGD are curated by BioGRID.

Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions

An interaction is defined as an experimentally observed physical or genetic interaction between two genes. There may be more than one row listed for the same interactor if the interaction between it and the given gene was observed in separate studies. All interactions listed in SGD are curated by BioGRID.

Physical Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Assay Annotation Action Modification Source Reference

Interaction Network

This diagram displays physical (purple lines) and genetic (green lines) interactions between the given gene (yellow circle) and its interactors (gray circles) based on the number of experiments supporting each interaction (adjustable using the slider at the bottom). Interactions between the interactors for a given gene are also shown.

Reset

Click on a gene to go to its specific page within SGD; drag any of the gene objects around within the visualization for easier viewing; filter by interaction type (genetic or physical) using the radio buttons; click “Reset” to automatically redraw the diagram; filter the number of interactors displayed by adjusting the number of experiments supporting a gene's interaction with the given gene by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


Resources

BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap

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