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Ribosomes are highly conserved large ribonucleoprotein (RNP) particles, consisting in yeast of a large 60S subunit and a small 40S subunit, that perform protein synthesis. Yeast ribosomes contain one copy each of four ribosomal RNAs (5S, 5.8S, 18S, and 25S; produced in two separate transcripts encoded within the rDNA repeat present as hundreds of copies on Chromosome 12) and 79 different ribosomal proteins (r-proteins), which are encoded by 137 different genes scattered about the genome, 59 of which are duplicated (7, 5). The 60S subunit contains 46 proteins and three RNA molecules: 25S RNA of 3392 nt, hydrogen bonded to the 5.8S RNA of 158 nt and associated with the 5S RNA of 121 nt. The 40S subunit has a single 18S RNA of 1798 nt and 33 proteins (8, 5). All yeast ribosomal proteins have a mammalian homolog (9).

In a rapidly growing yeast cell, 60% of total transcription is devoted to ribosomal RNA, and 50% of RNA polymerase II transcription and 90% of mRNA splicing are devoted to the production of mRNAs for r-proteins. Coordinate regulation of the rRNA genes and 137 r-protein genes is affected by nutritional cues and a number of signal transduction pathways that can abruptly induce or silence the ribosomal genes, whose transcripts have naturally short lifetimes, leading to major implications for the expression of other genes as well (10, 11, 12). The expression of some r-protein genes is influenced by Abf1p (13), and most are directly induced by binding of Rap1p to their promoters, which excludes nucleosomes and recruits Fhl1p and Ifh1p to drive transcription (14).

Ribosome assembly is a complex process, with different steps occurring in different parts of the cell. Ribosomal protein genes are transcribed in the nucleus, and the mRNA is transported to the cytoplasm for translation. The newly synthesized r-proteins then enter the nucleus and associate in the nucleolus with the two rRNA transcripts, one of which is methylated and pseudouridylated (view sites of modifications), and then cleaved into three individual rRNAs (18S, 5.8S, and 25S) as part of the assembly process (7). Separate ribosomal subunits are then transported from the nucleolus to the cytoplasm where they assemble into mature ribosomes before functioning in translation (15, 16). Blockage of subunit assembly, such as due to inhibition of rRNA synthesis or processing, results in degradation of newly synthesized r-proteins (17, 16). (For more information on the early steps of rRNA processing and small ribosomal subunit assembly, see the summary paragraph for the U3 snoRNA, encoded by snR17A and snR17B.)", "date_edited": "2007-02-14"}, "literature_overview": {"primary_count": 25, "additional_count": 51, "review_count": 7, "go_count": 3, "phenotype_count": 1, "disease_count": 0, "interaction_count": 68, "regulation_count": 9, "ptm_count": 12, "funComplement_count": 0, "htp_count": 29, "total_count": 185}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [], "URS_ID": null, "main_strain": "S288C", "regulation_overview": {"regulator_count": 14, "target_count": 0}, "reference_mapping": {"612181": 1, "592544": 2, "548623": 3, "526423": 4, "397935": 5, "2065584": 6, "592595": 7, "517471": 8, "556638": 9, "631529": 10, "554134": 11, "585697": 12, "608664": 13, "518051": 14, "592541": 15, "508692": 16, "508689": 17, "316136": 18}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: RPL24B", "date_created": "2000-05-19", "references": [{"id": 551590, "display_name": "SGD (2007)", "citation": "SGD (2007) Information without a citation in SGD ", "pubmed_id": null, "link": "/reference/S000073348", "year": 2007, "urls": []}]}, {"category": "Name", "history_type": "LSP", "note": "Name: L24e", "date_created": "2012-11-02", "references": [{"id": 397935, "display_name": "Jenner L, et al. (2012)", "citation": "Jenner L, et al. (2012) Crystal structure of the 80S yeast ribosome. Curr Opin Struct Biol 22(6):759-67", "pubmed_id": 22884264, "link": "/reference/S000150523", "year": 2012, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1016/j.sbi.2012.07.013"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/22884264"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: eL24", "date_created": "2016-08-09", "references": [{"id": 316136, "display_name": "Ban N, et al. (2014)", "citation": "Ban N, et al. (2014) A new system for naming ribosomal proteins. Curr Opin Struct Biol 24:165-9", "pubmed_id": 24524803, "link": "/reference/S000182084", "year": 2014, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1016/j.sbi.2014.01.002"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358319/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/24524803"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: rp29", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: L24B", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: L30B", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: YL21", "date_created": "2010-02-16", "references": [{"id": 592544, "display_name": "Planta RJ and Mager WH (1998)", "citation": "Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7", "pubmed_id": 9559554, "link": "/reference/S000058461", "year": 1998, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9559554"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: RPL30B", "date_created": "2010-02-16", "references": []}, {"category": "Annotation change", "history_type": "SEQUENCE", "note": "Annotation change: RPL24B/YGR148C mRNA contains an intron in the 5' untranslated region (UTR).", "date_created": "2007-04-04", "references": [{"id": 508230, "display_name": "Juneau K, et al. (2007)", "citation": "Juneau K, et al. (2007) High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing. Proc Natl Acad Sci U S A 104(5):1522-7", "pubmed_id": 17244705, "link": "/reference/S000120506", "year": 2007, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1073/pnas.0610354104"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1780280/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/17244705"}]}, {"id": 510418, "display_name": "Miura F, et al. (2006)", "citation": "Miura F, et al. (2006) A large-scale full-length cDNA analysis to explore the budding yeast transcriptome. Proc Natl Acad Sci U S A 103(47):17846-51", "pubmed_id": 17101987, "link": "/reference/S000119659", "year": 2006, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1073/pnas.0605645103"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1693835/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/17101987"}, {"display_name": "Reference supplement", "link": "http://www.pnas.org/cgi/content/full/0605645103/DC1"}, {"display_name": "Reference supplement", "link": "http://yeast.utgenome.org/"}]}, {"id": 536678, "display_name": "Mitra G and Warner JR (1984)", "citation": "Mitra G and Warner JR (1984) A yeast ribosomal protein gene whose intron is in the 5' leader. J Biol Chem 259(14):9218-24", "pubmed_id": 6086628, "link": "/reference/S000079790", "year": 1984, "urls": [{"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/6086628"}]}, {"id": 549853, "display_name": "Cliften P, et al. (2003)", "citation": "Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6", "pubmed_id": 12775844, "link": "/reference/S000073948", "year": 2003, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1126/science.1084337"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/12775844"}, {"display_name": "Reference supplement", "link": "http://www.sciencemag.org/cgi/content/full/1084337/DC1"}]}, {"id": 612181, "display_name": "Baronas-Lowell DM and Warner JR (1990)", "citation": "Baronas-Lowell DM and Warner JR (1990) Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae. Mol Cell Biol 10(10):5235-43", "pubmed_id": 2204809, "link": "/reference/S000051769", "year": 1990, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1128/mcb.10.10.5235-5243.1990"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC361207/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2204809"}]}]}], "complexes": []} RPL24B Disease | SGD

Disease Help

RPL24B / YGR148C Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature.


Manually Curated

Manually curated DO annotations reflect our best understanding of disease association for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated Disease annotations for accuracy and completeness.


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

High-throughput

DO annotations from high-throughput experiments are based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on DO annotations (or mappings to DO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator.


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

Computational

Computational DO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational DO annotations for S. cerevisiae are assigned by an external source (for example, the Alliance of Genome Resources (Alliance).


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

Shared Annotations

This diagram displays manually curated and high-throughput DO terms (orange circles) that are shared between the given gene (black circle), other yeast genes (dark blue circles), and human genes (light blue circles).


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