Once activated, Ste11p phosphorylates the correct target MAPKK, either Ste7p for the mating and filamentous growth pathways or Pbs2p for the osmosensing pathway, via interactions with the scaffolding proteins Ste5p and Pbs2p, respectively. (23, 24). During pheromone response, Ste5p tethers Ste11p, Ste7p, and either of the MAPKs Fus3p or Kss1p. During osmoregulation, Pbs2p brings together Sho1p, Ste11p, the MAPK Hog1p, and also serves as the MAPKK target (25, and reviewed in 26).Ste11p consists of a C-terminal kinase domain and three N-terminal regulatory domains: a sterile alpha motif (SAM) domain which binds to the Ste50p protein, a domain that interacts with Ste5p, followed by a catalytic-binding domain (CBD) that can bind and inhibit the activity of the C-terminus (reviewed in 8). Interaction of CBD with the catalytic domain is disrupted by the binding of Ste50p to the SAM domain and by Ste20p-mediated phosphorylation of serine and threonine residues in the CBD (9, 10). In response to pheromones, Ste11p is also regulated by ubiquitin-dependent protein degradation (13). There are some discrepancies in STE11 literature as to the exact length of the protein since there are three in-frame ATGs in the STE11 coding sequence. Originally it was thought that translation begins at the first ATG, but it was later shown that the third ATG is the actual start codon (2).", "date_edited": "2007-03-06"}, "literature_overview": {"primary_count": 170, "additional_count": 208, "review_count": 124, "go_count": 9, "phenotype_count": 21, "disease_count": 0, "interaction_count": 137, "regulation_count": 5, "ptm_count": 7, "funComplement_count": 0, "htp_count": 23, "total_count": 571}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [{"display_name": "2.7.11.25", "link": "/ecnumber/EC:2.7.11.25"}], "URS_ID": null, "main_strain": "S288C", "genetic_position": 244.0, "regulation_overview": {"regulator_count": 2, "target_count": 6}, "reference_mapping": {"614364": 1, "639690": 2, "629005": 3, "615164": 4, "546313": 5, "398398": 6, "536065": 7, "534164": 8, "536648": 9, "606090": 10, "512954": 11, "591122": 12, "560867": 13, "627802": 14, "529049": 15, "588917": 16, "626628": 17, "622556": 18, "522699": 19, "524851": 20, "525940": 21, "551124": 22, "611615": 23, "617848": 24, "563732": 25, "553503": 26}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: STE11", "date_created": "2000-05-19", "references": [{"id": 614364, "display_name": "Chaleff DT and Tatchell K (1985)", "citation": "Chaleff DT and Tatchell K (1985) Molecular cloning and characterization of the STE7 and STE11 genes of Saccharomyces cerevisiae. Mol Cell Biol 5(8):1878-86", "pubmed_id": 3915783, "link": "/reference/S000051032", "year": 1985, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1128/mcb.5.8.1878-1886.1985"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC366903/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/3915783"}]}]}, {"category": "Annotation change", "history_type": "SEQUENCE", "note": "Annotation change: The start site of YLR362W was moved 63 nucleotides downstream.", "date_created": "2006-04-17", "references": []}, {"category": "Mapping", "history_type": "SEQUENCE", "note": "Mapping: Edition 15: Note that there are three in-frame ATGs in the STE11 sequence. Orginally it was thought that the first ATG was the start, but Errede and colleagues have shown that the third ATG is the actual start codon (see Rhodes, N., et al. (1990) STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast. Genes Dev 4:1862-1874).", "date_created": "1998-11-10", "references": [{"id": 542517, "display_name": "Cherry JM, et al. (1998)", "citation": "Cherry JM, et al. (1998) \"Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 15)\". Pp. 414-420 in 1998 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America", "pubmed_id": null, "link": "/reference/S000076263", "year": 1998, "urls": []}]}, {"category": "Proposed annotation change", "history_type": "SEQUENCE", "note": "Proposed annotation change: Data from Nagalakshmi et al. 2008 suggest that there is an in-frame AUG 21 codons upstream of the currently annotated AUG for either all or some of the transcripts of STE11/YLR362W. The currently annotated start codon was experimentally verified by Rhodes, N. et al. (1990).", "date_created": "2008-11-05", "references": [{"id": 488779, "display_name": "Nagalakshmi U, et al. (2008)", "citation": "Nagalakshmi U, et al. (2008) The transcriptional landscape of the yeast genome defined by RNA sequencing. Science 320(5881):1344-9", "pubmed_id": 18451266, "link": "/reference/S000126260", "year": 2008, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1126/science.1158441"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2951732/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/18451266"}, {"display_name": "Reference supplement", "link": "http://www.sciencemag.org/cgi/content/full/1158441/DC1"}]}, {"id": 639690, "display_name": "Rhodes N, et al. (1990)", "citation": "Rhodes N, et al. (1990) STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast. Genes Dev 4(11):1862-74", "pubmed_id": 2276621, "link": "/reference/S000042489", "year": 1990, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1101/gad.4.11.1862"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2276621"}]}]}], "complexes": []},
tabs: {"id": 1283852, "protein_tab": true, "interaction_tab": true, "summary_tab": true, "go_tab": true, "sequence_section": true, "expression_tab": true, "phenotype_tab": true, "literature_tab": true, "wiki_tab": false, "regulation_tab": true, "sequence_tab": true, "history_tab": true, "homology_tab": true, "disease_tab": false}
};
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
BLASTN |
BLASTP |
Design Primers |
Restriction Fragment Map |
Restriction Fragment Sizes |
Six-Frame Translation
BLASTN vs. fungi |
BLASTP at NCBI |
BLASTP vs. fungi
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all STE11 alleles in SGD search
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
(Molecular Function,
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
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View computational annotations
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
298 total interactions for 130 unique genes
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-03-06
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.
STE11 / YLR362W Overview
Sequence
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Protein
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Molecular Function
Biological Process
Phenotype
Classical Genetics
Large-scale Survey
Interaction
Physical Interactions
Genetic Interactions
Regulation
Expression
Summary Paragraph
Literature
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