Scp160p is in close proximity to translation elongation factor 1A and the WD40 (Trp-Asp 40)-repeat containing protein Asc1p at ribosomes. The C-terminus of Scp160p is essential for ribosome binding, and this interaction depends on Asc1p. It has been suggested that Scp160p connects specific mRNAs, ribosomes, and a translation factor with an adaptor for signalling molecules. These interactions might regulate the translation activity of ribosomes programmed with specific mRNAs (2).Scp160p is also an essential component of the mating response pathway and is the first RNA-binding protein to be indentified as a G protein effector. Scp160p binds the Gpa1p GTPase and signaling by activated Gpa1p requires this direct coupling to Scp160p (4). SCP160 interacts genetically and biochemically with EAP1, whose protein product functions in translation as an eIF4E-binding protein with additional uncharacterized spindle pole body functions, confirming that Scp160p plays a role in translation. Loss of either gene results in significant changes in either the complex associations or subcellular distribution of the other protein (9). Disruption of SCP160 also results in decreased viability, abnormal morphology, and increased DNA content, a complex phenotype that is not reversible by transformation with a plasmid carrying the wild-type gene (1). Further, loss of Scp160p results in changes in both the abundance and distribution between soluble and membrane-associated fractions for some messages (YOR338W), and in a subtle shift from soluble polyribosomes to soluble mRNPs for others (DHH1 and YOR338W) (11).", "date_edited": "2005-03-30"}, "literature_overview": {"primary_count": 39, "additional_count": 33, "review_count": 10, "go_count": 7, "phenotype_count": 6, "disease_count": 0, "interaction_count": 83, "regulation_count": 8, "ptm_count": 14, "funComplement_count": 0, "htp_count": 63, "total_count": 210}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [], "URS_ID": null, "main_strain": "S288C", "regulation_overview": {"regulator_count": 8, "target_count": 0}, "reference_mapping": {"604394": 1, "539927": 2, "537463": 3, "549288": 4, "622406": 5, "629787": 6, "398901": 7, "588648": 8, "546048": 9, "587409": 10, "552795": 11}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: SCP160", "date_created": "2000-05-19", "references": [{"id": 604394, "display_name": "Wintersberger U, et al. (1995)", "citation": "Wintersberger U, et al. (1995) Scp160p, a new yeast protein associated with the nuclear membrane and the endoplasmic reticulum, is necessary for maintenance of exact ploidy. Yeast 11(10):929-44", "pubmed_id": 8533468, "link": "/reference/S000054401", "year": 1995, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/yea.320111004"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/8533468"}]}]}], "complexes": []},
tabs: {"id": 1285239, "protein_tab": true, "interaction_tab": true, "summary_tab": true, "go_tab": true, "sequence_section": true, "expression_tab": true, "phenotype_tab": true, "literature_tab": true, "wiki_tab": false, "regulation_tab": true, "sequence_tab": true, "history_tab": true, "homology_tab": true, "disease_tab": false}
};
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
BLASTN |
BLASTP |
Design Primers |
Restriction Fragment Map |
Restriction Fragment Sizes |
Six-Frame Translation
BLASTN vs. fungi |
BLASTP at NCBI |
BLASTP vs. fungi
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SCP160 alleles in SGD search
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
(Molecular Function,
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
View computational annotations
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
346 total interactions for 286 unique genes
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-03-30
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.
SCP160 / YJL080C Overview
Sequence
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Protein
Alleles
Gene Ontology
Molecular Function
Biological Process
Cellular Component
Phenotype
Classical Genetics
Large-scale Survey
Interaction
Physical Interactions
Genetic Interactions
Regulation
Expression
Summary Paragraph
Literature
Resources