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CTT1 expression is induced under various stresses, such as carbon starvation and oxidative, osmotic, or freeze-thaw stress (reviewed in 12). This transcriptional response is mediated by transcription factors such as Msn2p/Msn4p, Hog1p, Hap1p, Yap1p, and Zap1p acting upon upstream activating elements (UAS) in the CTT1 promoter, including the stress response element (STRE) and a heme control region (13, 14, 15, 16, 8, 17). The localization of Ctt1p is regulated during cytokinesis, with a higher proportion of active Ctt1p found in daughter cells (18).

The S. cerevisiae genome encodes a second catalase, peroxisomal catalase A (encoded by CTA1), and although these two enzymes are functionally similar, Cta1p is more similar in sequence to the peroxiosomal catalases from the cow Bos taurus and the pathogenic yeast Candida tropicalis than it is to Ctt1p (19). All yeast species appear to have a Cta1p ortholog however not all yeasts have an ortholog of Ctt1p (20).", "date_edited": "2010-02-10"}, "literature_overview": {"primary_count": 125, "additional_count": 303, "review_count": 64, "go_count": 4, "phenotype_count": 18, "disease_count": 0, "interaction_count": 32, "regulation_count": 15, "ptm_count": 3, "funComplement_count": 0, "htp_count": 19, "total_count": 537}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [{"display_name": "1.11.1.6", "link": "/ecnumber/EC:1.11.1.6"}], "URS_ID": null, "main_strain": "S288C", "regulation_overview": {"regulator_count": 27, "target_count": 0}, "reference_mapping": {"631562": 1, "605779": 2, "644942": 3, "540055": 4, "582531": 5, "608944": 6, "531369": 7, "610981": 8, "531898": 9, "531207": 10, "628516": 11, "492608": 12, "642979": 13, "644272": 14, "628576": 15, "484746": 16, "634278": 17, "500164": 18, "620908": 19, "471268": 20, "506055": 21}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: CTT1", "date_created": "2000-05-19", "references": [{"id": 631562, "display_name": "Traczyk A, et al. (1985)", "citation": "Traczyk A, et al. (1985) Catalase T deficient mutants of Saccharomyces cerevisiae. Acta Microbiol Pol 34(3-4):231-41", "pubmed_id": 2421539, "link": "/reference/S000045230", "year": 1985, "urls": [{"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2421539"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: SPS101", "date_created": "2010-02-16", "references": [{"id": 506055, "display_name": "Neiman A (2007)", "citation": "Neiman A (2007) ", "pubmed_id": null, "link": "/reference/S000121298", "year": 2007, "urls": []}]}, {"category": "Annotation change", "history_type": "SEQUENCE", "note": "Annotation change: Based on based on 5' SAGE TSS and multiple orthologous sequences alignment, Zhang and Dietrich suggest that the start site CTT1/YGR088W be moved 33 nt (11 codons) downstream. This is the same start codon published in the initial characterization by Hartig and Ruis. This change has been incorporated into SGD and the numbering for both the nucleotides in the DNA coding sequence and the amino acids in the predicted protein have been changed accordingly.", "date_created": "2006-01-23", "references": [{"id": 531165, "display_name": "Zhang Z and Dietrich FS (2005)", "citation": "Zhang Z and Dietrich FS (2005) Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE. Nucleic Acids Res 33(9):2838-51", "pubmed_id": 15905473, "link": "/reference/S000082006", "year": 2005, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1093/nar/gki583"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131933/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/15905473"}, {"display_name": "Reference supplement", "link": "http://data.cgt.duke.edu/5sage.php"}]}, {"id": 607155, "display_name": "Hartig A and Ruis H (1986)", "citation": "Hartig A and Ruis H (1986) Nucleotide sequence of the Saccharomyces cerevisiae CTT1 gene and deduced amino-acid sequence of yeast catalase T. Eur J Biochem 160(3):487-90", "pubmed_id": 3536508, "link": "/reference/S000053472", "year": 1986, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1111/j.1432-1033.1986.tb10065.x"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/3536508"}]}]}, {"category": "Proposed annotation change", "history_type": "SEQUENCE", "note": "Proposed annotation change: RNA sequencing data from Nagalakshmi et al. 2008 suggest that there is an in-frame AUG 11 codons upstream of the currently annotated AUG for either all or some of the transcripts of CTT1/YGR088W. The upstream AUG represents the previously annotated start for this ORF, the annotation of which was changed based on 5' SAGE data from Zhang & Dietrich 2005, along with data from the initial characterization of the gene by Hartig & Ruis 1986.", "date_created": "2008-10-24", "references": [{"id": 488779, "display_name": "Nagalakshmi U, et al. (2008)", "citation": "Nagalakshmi U, et al. (2008) The transcriptional landscape of the yeast genome defined by RNA sequencing. Science 320(5881):1344-9", "pubmed_id": 18451266, "link": "/reference/S000126260", "year": 2008, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1126/science.1158441"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2951732/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/18451266"}, {"display_name": "Reference supplement", "link": "http://www.sciencemag.org/cgi/content/full/1158441/DC1"}]}, {"id": 531165, "display_name": "Zhang Z and Dietrich FS (2005)", "citation": "Zhang Z and Dietrich FS (2005) Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE. Nucleic Acids Res 33(9):2838-51", "pubmed_id": 15905473, "link": "/reference/S000082006", "year": 2005, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1093/nar/gki583"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131933/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/15905473"}, {"display_name": "Reference supplement", "link": "http://data.cgt.duke.edu/5sage.php"}]}, {"id": 607155, "display_name": "Hartig A and Ruis H (1986)", "citation": "Hartig A and Ruis H (1986) Nucleotide sequence of the Saccharomyces cerevisiae CTT1 gene and deduced amino-acid sequence of yeast catalase T. Eur J Biochem 160(3):487-90", "pubmed_id": 3536508, "link": "/reference/S000053472", "year": 1986, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1111/j.1432-1033.1986.tb10065.x"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/3536508"}]}]}], "complexes": []}, tabs: {"id": 1268455, "protein_tab": true, "interaction_tab": true, "summary_tab": true, "go_tab": true, "sequence_section": true, "expression_tab": true, "phenotype_tab": true, "literature_tab": true, "wiki_tab": false, "regulation_tab": true, "sequence_tab": true, "history_tab": true, "homology_tab": true, "disease_tab": false} }; CTT1 | SGD

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CTT1 / YGR088W Overview

Standard Name
CTT1 1
Systematic Name
YGR088W
SGD ID
SGD:S000003320
Aliases
SPS101 21
Feature Type
ORF , Verified
Description
Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide 2
Name Description
CaTalase T 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Analyze Sequence

S288C only

BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation

S288C vs. other species

BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi

S288C vs. other strains

Strain Alignment | Variant Viewer

Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
562
Mol. Weight (Da)
64575.9
Isoelectric Point
6.51
Median Abundance (molecules/cell)
4910 +/- 3463
Half-life (hr)
108.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all CTT1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Summary
Cytoplasmic catalase involved in response to reactive oxygen species

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways

Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Summary
Non-essential gene in reference strain S288C; null mutant has lengthened chronological lifespan; null mutant is sensitive to desiccation, heat shock, oxidative stress, and various chemicals; null mutant exhibits increased formation of the [Psi+] prion; in large-scale studies competitive fitness of the null mutant is reduced in minimal media but increased in glycerol- or ethanol-containing media

Classical Genetics

null
overexpression

Large-scale Survey

null
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

87 total interactions for 73 unique genes

Physical Interactions

  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 3
  • Co-localization: 1
  • Proximity Label-MS: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Rescue: 6
  • Negative Genetic: 35
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 7
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Regulators
27
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2010-02-10

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.

Primary
125
Additional
303
Reviews
64

Resources

AlphaFold Protein Structure | E.C.1.11.1.6 | Entrez Gene | Entrez RefSeq Protein | FungiDB | Search all NCBI | UniParc | UniProtKB