Pma1p is highly regulated by glucose, both transcriptionally and postranslationally (13). The presence of glucose induces a 2- to 4-fold increase in PMA1 expression, mediated by the transcription factors Rap1p and Gcr1p (14, 15). Glucose also triggers a rapid 5- to 10-fold increase in ATPase catalytic activity via Ptk2p-mediated phosphorylation of a serine residue in the Pma1p C-terminus (13, 16, 17). In contrast, phosphorylation of Pma1p by serine/threonine kinase (Yck1p and Yck2p), in the presence of glucose, is correlated with decreased proton pump activity (18). Glucose addition induces a Pma1p conformational change that is correlated with enzyme activity; moreover, glucose disrupts complexes between acetylated tubulin (Tub1p, Tub2p, and Tub3p) and Pma1p that are known to inhibit H+-ATPase activity (19 and references contained therein, 20). Finally, PMA1 transcription may be regulated by the cell cycle as its promoter is also a binding site for the transcription factor Mcm1p (21). Pma1p homologs have been identified in A. thaliana and other fungi such as C. albicans and S. pombe (22, 23, 24). Additionally, H+-ATPase homologs in pathogenic fungi are being studied as targets for antifungal reagents (25 and reviewed in 26).", "date_edited": "2006-04-27"}, "literature_overview": {"primary_count": 279, "additional_count": 308, "review_count": 99, "go_count": 12, "phenotype_count": 10, "disease_count": 0, "interaction_count": 148, "regulation_count": 13, "ptm_count": 18, "funComplement_count": 0, "htp_count": 23, "total_count": 827}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [{"display_name": "7.1.2.1", "link": "/ecnumber/EC:7.1.2.1"}], "URS_ID": null, "main_strain": "S288C", "genetic_position": -2.0, "regulation_overview": {"regulator_count": 25, "target_count": 0}, "reference_mapping": {"592214": 1, "576647": 2, "615179": 3, "610722": 4, "416907": 5, "418282": 6, "362735": 7, "362207": 8, "644981": 9, "590599": 10, "524494": 11, "560753": 12, "576653": 13, "617452": 14, "631775": 15, "523996": 16, "592151": 17, "635631": 18, "525395": 19, "526367": 20, "615897": 21, "576567": 22, "615312": 23, "576662": 24, "576573": 25, "576588": 26, "648276": 27}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: KTI10", "date_created": "2010-02-16", "references": [{"id": 648276, "display_name": "Butler AR, et al. (1994)", "citation": "Butler AR, et al. (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16", "pubmed_id": 8065362, "link": "/reference/S000039420", "year": 1994, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1128/mcb.14.9.6306-6316.1994"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC359157/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/8065362"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: PMA1", "date_created": "2000-05-19", "references": [{"id": 592214, "display_name": "McCusker JH (1987)", "citation": "McCusker JH (1987) Pleiotropic drug resistance mutations in Saccharomyces cerevisiae. Ph.D. Thesis", "pubmed_id": null, "link": "/reference/S000058967", "year": 1987, "urls": []}]}, {"category": "Mapping", "history_type": "SEQUENCE", "note": "Mapping: Edition 10: cen7-pma1 physical distance is 35 kb", "date_created": "1989-10-01", "references": [{"id": 552023, "display_name": "Mortimer RK, et al. (1989)", "citation": "Mortimer RK, et al. (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5(5):321-403", "pubmed_id": 2678811, "link": "/reference/S000073208", "year": 1989, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/yea.320050503"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2678811"}]}]}, {"category": "Mapping", "history_type": "SEQUENCE", "note": "Mapping: Edition 10: Physical map trp5 to pma1 (10 ORF including 4 FUN genes are located in this interval)", "date_created": "1989-10-01", "references": [{"id": 552023, "display_name": "Mortimer RK, et al. (1989)", "citation": "Mortimer RK, et al. (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5(5):321-403", "pubmed_id": 2678811, "link": "/reference/S000073208", "year": 1989, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/yea.320050503"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2678811"}]}]}], "complexes": []},
tabs: {"id": 1269045, "protein_tab": true, "interaction_tab": true, "summary_tab": true, "go_tab": true, "sequence_section": true, "expression_tab": true, "phenotype_tab": true, "literature_tab": true, "wiki_tab": false, "regulation_tab": true, "sequence_tab": true, "history_tab": true, "homology_tab": true, "disease_tab": false}
};
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
BLASTN |
BLASTP |
Design Primers |
Restriction Fragment Map |
Restriction Fragment Sizes |
Six-Frame Translation
BLASTN vs. fungi |
BLASTP at NCBI |
BLASTP vs. fungi
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PMA1 alleles in SGD search
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
(Molecular Function,
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
View computational annotations
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
1004 total interactions for 839 unique genes
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-04-27
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.
PMA1 / YGL008C Overview
Sequence
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Protein
Alleles
Gene Ontology
Molecular Function
Biological Process
Cellular Component
Phenotype
Classical Genetics
Large-scale Survey
Interaction
Physical Interactions
Genetic Interactions
Regulation
Expression
Summary Paragraph
Literature
Resources