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One traditional analysis has shown that a pig2 null mutant in the EG328-1A strain background accumulates normal levels of glycogen (1). However, in a large-scale study using a homozygous diploid deletion series in the BY4743 strain background, a pig2 null mutant accumulates less glycogen than wild type (4). glc7-109 mutants display increased levels of glycogen, and this hyperglycogen phenotype is partially retained in pig1 gac1 gip2 triple null glc7-109 mutants, indicating that Pig2p may be a redundant targeting subunit capable of targeting Glc7p-109 to Gsy2p (2).

Gac1p, Pig1p, Pig2p and Gip2p are the only four yeast proteins that share a conserved segment of 25 residues, designated the GVNK motif, which is also found in mammalian type-1 protein phosphatase targeting subunits (1). Pig2p also possesses two short regions of similarity to the Rhizopus glucoamylase precursor AMYG, and to various mammalian glycogen-associated regulatory subunits of protein phosphatase 1 (1), including rabbit RG RAB and human PPP1R3, mutations in which are associated with non-insulin-dependent diabetes mellitus (5).", "date_edited": "2005-10-25"}, "literature_overview": {"primary_count": 5, "additional_count": 11, "review_count": 1, "go_count": 2, "phenotype_count": 2, "disease_count": 0, "interaction_count": 24, "regulation_count": 4, "ptm_count": 9, "funComplement_count": 0, "htp_count": 19, "total_count": 61}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [], "URS_ID": null, "main_strain": "S288C", "regulation_overview": {"regulator_count": 6, "target_count": 0}, "reference_mapping": {"643736": 1, "562088": 2, "526423": 3, "560965": 4, "643484": 5}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: PIG2", "date_created": "2000-05-19", "references": [{"id": 643736, "display_name": "Cheng C, et al. (1997)", "citation": "Cheng C, et al. (1997) Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p. Yeast 13(1):1-8", "pubmed_id": 9046081, "link": "/reference/S000041126", "year": 1997, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/(SICI)1097-0061(199701)13:1<1::AID-YEA49>3.0.CO;2-F"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/9046081"}]}]}], "complexes": []}, tabs: {"id": 1266886, "protein_tab": true, "interaction_tab": true, "summary_tab": true, "go_tab": true, "sequence_section": true, "expression_tab": true, "phenotype_tab": true, "literature_tab": true, "wiki_tab": false, "regulation_tab": true, "sequence_tab": true, "history_tab": true, "homology_tab": true, "disease_tab": false} }; PIG2 | SGD

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PIG2 / YIL045W Overview

Standard Name
PIG2 1
Systematic Name
YIL045W
SGD ID
SGD:S000001307
Feature Type
ORF , Verified
Description
Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication 1 2 3
Name Description
Protein Interacting with Gsy2p 1
Paralog
GIP2 3
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Summary
PIG2 has a paralog, GIP2, that arose from the whole genome duplication

Analyze Sequence

S288C only

BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation

S288C vs. other species

BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi

S288C vs. other strains

Strain Alignment | Variant Viewer

Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
538
Mol. Weight (Da)
61942.2
Isoelectric Point
8.82
Median Abundance (molecules/cell)
1259 +/- 399

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all PIG2 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Summary
Regulatory subunit of the type 1 protein phosphatase involved in the regulation of glycogen biosynthesis; localizes to the cytoplasm

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Summary
The pig2 null mutant is viable; the null mutant of paralog gip2 is viable; the pig2 gip2 double mutant has not been annotated for phenotype.

74 total interactions for 66 unique genes

Physical Interactions

  • Affinity Capture-MS: 21
  • Affinity Capture-RNA: 6
  • Co-localization: 1
  • Two-hybrid: 5

Genetic Interactions

  • Dosage Growth Defect: 1
  • Negative Genetic: 35
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Regulators
6
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2005-10-25

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.

Primary
5
Additional
11
Reviews
1

Resources

AlphaFold Protein Structure | Entrez Gene | Entrez RefSeq Protein | FungiDB | Search all NCBI | UniParc | UniProtKB