Tup1p-Cyc8p is recruited to target genes by interaction with DNA-bound transcriptional repressors that recognize specific sequences within the target gene promoters. Such repressors include MatAlpha2p, which regulates mating-type-specific genes (23), Mig1p, which regulates glucose-repressed genes (24), Rfx1p, which is involved in DNA repair (25), and Sko1p, which is involved in stress responses (26). This gene-specific role has been complemented by observations that Tup1p might be involved in establishing domains of heterochromatin structure in the subtelomeric regions of chromosomes (27). These \"HAST\" domains contain clusters of Tup1p- and Cyc8p-repressed genes and coincide with regions that are deacetylated by the histone deacetylase Hda1p. Therefore, Tup1p-Cyc8p may establish the formation of heterochromatin in these regions by recruiting Hda1p. HAST domains are distinct from adjacent heterochromatin regions that are established via Tup1p-independent recruitment of the Sir2p histone deacetylase (28).", "date_edited": "2005-06-28"}, "literature_overview": {"primary_count": 146, "additional_count": 136, "review_count": 98, "go_count": 11, "phenotype_count": 12, "disease_count": 0, "interaction_count": 101, "regulation_count": 15, "ptm_count": 16, "funComplement_count": 0, "htp_count": 37, "total_count": 459}, "disease_overview": {"manual_disease_terms": [], "htp_disease_terms": [], "computational_annotation_count": 0, "date_last_reviewed": null}, "ecnumbers": [], "URS_ID": null, "main_strain": "S288C", "genetic_position": 56.0, "regulation_overview": {"regulator_count": 7, "target_count": 214, "paragraph": {"text": "Cyc8p (also known as Ssn6p) acts with its partner Tup1p as a corepressor to regulate the expression of multiple genes under a variety of conditions. Tup1p-Cyc8p does not bind to DNA directly but is recruited to specific promoters by DNA binding proteins such as Alpha2p, Mig1p, and Crt1p. Evidence suggests that Tup1p-Cyc8p represses transcription by masking and inhibiting the transcriptional activation domains of the recruiting proteins. Tup1p interacts directly with histones H3 and H4, and mutation of these histones synergistically compromises Tup1p-Cyc8p-mediated repression. Histone hyperacetylation caused by combined mutations in genes encoding the histone deacetylases Rpd3p, Hos1p, and Hos2p abolishes repression by Tup1p-Cyc8p. Regulatory regions bound by Tup1p assume a distinct chromatin architecture including nucleosome-depleted regions, poorly positioned promoter nucleosomes and a larger number and wider distribution of accessible transcription factor binding sites. The Tup1p-Cyc8p corepressor complex is composed of one Cyc8p and four Tup1p subunits.", "date_edited": "2016-10-15", "references": [{"id": 634168, "display_name": "Varanasi US, et al. (1996)", "citation": "Varanasi US, et al. (1996) The Cyc8 (Ssn6)-Tup1 corepressor complex is composed of one Cyc8 and four Tup1 subunits. Mol Cell Biol 16(12):6707-14", "pubmed_id": 8943325, "link": "/reference/S000044346", "year": 1996, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1128/MCB.16.12.6707"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC231673/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/8943325"}]}, {"id": 590384, "display_name": "Watson AD, et al. (2000)", "citation": "Watson AD, et al. (2000) Ssn6-Tup1 interacts with class I histone deacetylases required for repression. Genes Dev 14(21):2737-44", "pubmed_id": 11069890, "link": "/reference/S000059687", "year": 2000, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1101/gad.829100"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC317033/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/11069890"}]}, {"id": 408128, "display_name": "Wong KH and Struhl K (2011)", "citation": "Wong KH and Struhl K (2011) The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein. Genes Dev 25(23):2525-39", "pubmed_id": 22156212, "link": "/reference/S000147776", "year": 2011, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1101/gad.179275.111"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243062/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/22156212"}]}, {"id": 412703, "display_name": "Rizzo JM, et al. (2011)", "citation": "Rizzo JM, et al. (2011) Tup1 stabilizes promoter nucleosome positioning and occupancy at transcriptionally plastic genes. Nucleic Acids Res 39(20):8803-19", "pubmed_id": 21785133, "link": "/reference/S000146190", "year": 2011, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1093/nar/gkr557"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203618/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/21785133"}]}]}}, "reference_mapping": {"596094": 1, "637880": 2, "560459": 3, "476522": 4, "2557751": 5, "548194": 6, "546677": 7, "560405": 8, "631022": 9, "634168": 10, "647746": 11, "556300": 12, "560510": 13, "648825": 14, "646251": 15, "642916": 16, "590384": 17, "589503": 18, "546816": 19, "563601": 20, "584898": 21, "584901": 22, "619083": 23, "599862": 24, "647160": 25, "548051": 26, "560693": 27, "533086": 28, "619366": 29, "601211": 30}, "history": [{"category": "Name", "history_type": "LSP", "note": "Name: CRT8", "date_created": "2010-02-16", "references": [{"id": 619366, "display_name": "Zhou Z and Elledge SJ (1992)", "citation": "Zhou Z and Elledge SJ (1992) Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131(4):851-66", "pubmed_id": 1516817, "link": "/reference/S000049348", "year": 1992, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1093/genetics/131.4.851"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1205097/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/1516817"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: CYC8", "date_created": "2000-05-19", "references": [{"id": 596094, "display_name": "Rothstein RJ and Sherman F (1980)", "citation": "Rothstein RJ and Sherman F (1980) Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions. Genetics 94(4):871-89", "pubmed_id": 6254831, "link": "/reference/S000057232", "year": 1980, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1093/genetics/94.4.871"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214186/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/6254831"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: [OCT]", "date_created": "2011-03-15", "references": []}, {"category": "Name", "history_type": "LSP", "note": "Name: [OCT1+]", "date_created": "2011-03-15", "references": [{"id": 476522, "display_name": "Patel BK, et al. (2009)", "citation": "Patel BK, et al. (2009) The yeast global transcriptional co-repressor protein Cyc8 can propagate as a prion. Nat Cell Biol 11(3):344-9", "pubmed_id": 19219034, "link": "/reference/S000129327", "year": 2009, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1038/ncb1843"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667906/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/19219034"}]}]}, {"category": "Name", "history_type": "LSP", "note": "Name: SSN6", "date_created": "2010-02-16", "references": [{"id": 601211, "display_name": "Schultz J and Carlson M (1987)", "citation": "Schultz J and Carlson M (1987) Molecular analysis of SSN6, a gene functionally related to the SNF1 protein kinase of Saccharomyces cerevisiae. Mol Cell Biol 7(10):3637-45", "pubmed_id": 3316983, "link": "/reference/S000055475", "year": 1987, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1128/mcb.7.10.3637-3645.1987"}, {"display_name": "PMC full text", "link": "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC368018/"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/3316983"}]}]}, {"category": "Mapping", "history_type": "SEQUENCE", "note": "Mapping: Edition 10: cyc8 and ssn6 are allelic", "date_created": "1989-10-01", "references": [{"id": 552023, "display_name": "Mortimer RK, et al. (1989)", "citation": "Mortimer RK, et al. (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5(5):321-403", "pubmed_id": 2678811, "link": "/reference/S000073208", "year": 1989, "urls": [{"display_name": "DOI full text", "link": "http://dx.doi.org/10.1002/yea.320050503"}, {"display_name": "PubMed", "link": "http://www.ncbi.nlm.nih.gov/pubmed/2678811"}]}]}], "complexes": [{"format_name": "CPX-1663", "display_name": "CYC8-TUP1 corepressor complex"}]},
tabs: {"id": 1267370, "protein_tab": true, "interaction_tab": true, "summary_tab": true, "go_tab": true, "sequence_section": true, "expression_tab": true, "phenotype_tab": true, "literature_tab": true, "wiki_tab": false, "regulation_tab": true, "sequence_tab": true, "history_tab": true, "homology_tab": true, "disease_tab": false}
};
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
BLASTN |
BLASTP |
Design Primers |
Restriction Fragment Map |
Restriction Fragment Sizes |
Six-Frame Translation
BLASTN vs. fungi |
BLASTP at NCBI |
BLASTP vs. fungi
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CYC8 alleles in SGD search
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
(Molecular Function,
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
View computational annotations
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
295 total interactions for 194 unique genes
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-06-28
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.
CYC8 / YBR112C Overview
Sequence
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Protein
Alleles
Gene Ontology
Molecular Function
Biological Process
Cellular Component
Complex
Phenotype
Classical Genetics
Large-scale Survey
Interaction
Physical Interactions
Genetic Interactions
Regulation
Expression
Summary Paragraph
Literature
Resources