Since the term nanobiosensor first emerged over three decades ago, the field has witnessed an explosion of groundbreaking research. Thanks to the development of advanced nanomaterials and nanotechnologies, combined with decades of expertise in biosensing, a wide range of innovative and improved nanobiosensors have been reported, but many challenges remain. For this technology to truly meet real-world needs â particularly in global health and related applications â further efforts are needed to improve performance, useability, scalability and cost-effectiveness.