Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice
Figure 8
Depletion of circulating monocytes reverses tissue protection in tsp-1−/− mice.
Histological analysis of gastrocnemius muscle sections at d4 after ischemia under Clo-Lip treatment. (A–D) show H&E staining. (E–H) and (I–L) show immunostainings of adjacent sections for macrophages using Mac-3 Ab, and endothelial cells using CD31 Ab, respectively. (A, B; E, F; I, J) scale bar = 500 µm; (C, D; G, H; K, L) scale bar = 200 µm. (M) Quantification of histologically different area types ratio; mean±S.E.M. are shown, n = 5. (N) Proportion of mice exhibiting macroscopic necrosis under Clo-Lip treatment, d4 after ischemia. * = p<0.05, n = 10. (O) Quantification of regenerating basic myofibers in necrotic areas, mean±S.E.M. are shown, * = p<0.05, n = 10. (P) Quantification of capillary density under Clo-Lip treatment in the different area types in gastrocnemius muscles, d4 after ischemia; Pbs-Lip injected mice served as control. Mean±SEM are shown. * = p<0.05; n = 5.