Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice
Figure 7
Thrombospondin-1−/− mice exhibit a less pro-inflammatory macrophage activation state in response to ischemia.
(A) FACS analyses of CD45+ cells isolated from ischemic muscles at d4, stained for F4/80 and Ly-6C expression, wt, left panel; tsp-1−/−, right panel; n = 5 mice per group. (B) Quantification of F4/80(lo)Ly-6C(hi), F4/80(hi)Ly-6C(hi) and F4/80(hi)Ly-6C(lo) macrophage proportions in CD45+ cells isolated from ischemic muscles at d4 in both genotypes (n = 5 mice per group in five independent experiments). * = p<0,05 vs. wt. (C) Quantitative RT-PCR analyses of IL-1β, TNF-α, IL-10, PPAR-γ, TGF-β, iNOS and Arg1 expression in F4/80(hi) vs. F4/80(lo)Ly-6C(hi) cells isolated from ischemic muscles at d4 in both genotypes.