Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice
Figure 1
Thrombospondin-1 is expressed during critical hind limb ischemia in mice.
Thrombospondin-1 mRNA expression was analyzed in gastrocnemius muscle using in situ hybridization at d4 (D), d6 (F), d16 (H) and d21 (J) after ischemia. D0 (B) represents non ischemic tissue. Adjacent sections (respectively C, E, G, I and A were stained with H&E (Scale bar = 200 µm). (L) Gastrocnemius muscle analyzed at d4 for tsp-1 mRNA expression at a higher magnification (scale bar = 25 µm). (K, M) Adjacent sections were stained for macrophages (K) and endothelial cells (M), showing expression of tsp-1 mRNA in macrophages (see arrows in K&L) and endothelial cells (see arrowheads in L&M). Arrowheads in H&J show tsp-1 mRNA expression in endothelial cells at d16 and d21 respectively. (N) Western blot analysis of TSP-1 expression in gastrocnemius muscle at similar time points. Arrowhead = 150 kD.