Survival of the Replication Checkpoint Deficient Cells Requires MUS81-RAD52 Function
Figure 5
MUS81 and RAD52 differently affect restart of stalled forks upon CHK1 inhibition.
(A) GM01604 cells were transfected with siRNAs directed against GFP (siCtrl), MUS81 (siMUS81) or RAD52 (siRAD52). Replication sites were first labeled with CldU (red signal), left untreated or treated as indicated, followed by recovery for 45 min in IdU (green signal). After immunostaining with antibodies specific for CldU and IdU, the overlapping foci were quantified in each isolated red-positive cell and results expressed as the percentage of CldU/IdU colocalising foci (0–20% of total CldU foci; 20–60% of total CldU foci; 60–100% of total CldU foci). Data are presented as percentage of dead cells and are mean of three independent experiments. Error bars represent standard error. The images shown in the panel (B) are representative of labeling and of different classes of colocalising nuclei.