Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification
Figure 8
Sir4 protein levels are decreased in the elp3Δ mutant.
(A). Western blot analysis of Sir4-Myc protein levels in the elp3Δ strain transformed with plasmids pRS315-ELP3, pRS425-tK-tQ-tE or pRS425. The ratios of Sir4-Myc to Actin signals were calculated. The values are shown relative to elp3Δ pRS315-ELP3 strain, which was arbitrarily set to 1, and are the average of two independent experiments. (B). Northern blot analysis of SIR4 mRNA. The elp3Δ strain was transformed with plasmids pRS315-ELP3, pRS425-tK-tQ-tE or pRS425. Signals of SIR4 mRNAs were normalized to the non-coding SCR1 transcript. The values are shown relative to elp3Δ pRS315-ELP3 strain, which was arbitrarily set to 1, and are the average of two independent experiments. (C). The wild type strain (UMY2584) transformed with plasmids pRS424-SIR4 or pRS424, and the elp3Δ mutant (UMY3790) with plasmids pRS424-SIR4 or pRS424 were assayed as described in Figure 1. For A, B and C, representative figures are shown. Abbreviations for the tRNA genes encoding ,
and
are tK, tQ and tE, respectively.