Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification
Figure 6
U34 modification levels influence ochre stop codon read through by a suppressor tRNA.
(A) Schematic drawing of the dual luciferase reporter system constructed by Keeling et al [27]. The sequence of the read through cassette between Renilla and firefly luciferase genes is shown. The XXX in red stands for either UAA in the assay plasmid or CAA in the control plasmid. (B) Read through levels of the UAA stop codon in SUP4 (UMY2894), elp3-C103A SUP4 (UMY3314), elp3-G168R SUP4 (UMY3794), elp3-Y541A SUP4 (UMY3060), elp3-Y541A SUP4 (UMY3061) and elp3Δ SUP4 (UMY2915). Values are ratios of Firefly to Renilla luciferase activities and based on three independent experiments. The error bars represent the standard deviation. Values were normalized to the wild type SUP4 (UMY2894), which was arbitrarily set to 1.