Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification
Figure 5
HPLC analysis of total tRNAs isolated from mutants with different alleles of ELP3.
HPLC chromatograms of total tRNA isolated from SUP4 (UMY2894), elp3Δ SUP4 (UMY2915), elp3-C103A SUP4 (UMY3314), elp3-C108A SUP4 (UMY3315), elp3-C118A SUP4 (UMY3316), elp3-C121A SUP4 (UMY3317), elp3-G168R SUP4 (UMY3794), elp3-G180R G181R SUP4 (UMY3795), elp3-Y540A SUP4 (UMY3060) and elp3-Y541A SUP4 (UMY3061). Chromatograms were monitored at 314 nm. The parts of chromatograms between retention times 43 and 52 min are displayed. The arrows in B, D, E, F and H indicate the expected retention time of mcm5s2U.