Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification
Figure 4
Strains carrying different ELP3 mutant alleles show decreased telomeric gene silencing and increased HU sensitivity.
(A) Schematic drawing of the protein structure of Elp3. Orange box represents the radical-S-adenosyl methionine (Radical-SAM) domain and blue box indicates the location of the histone acetyltransferase (HAT) domain. Cysteine residues at position 103, 108, 118 or 121 were substituted with alanines. Glycine residues at position 168 or 180 together with 181 were replaced by arginines. Two tyrosine residues, positions 540 or 541, in the HAT domain were substituted with alanines. (B) The wild type and the different mutant alleles of the ELP3 gene, located in LEU2 containing vector pRS315, were transformed into the elp3Δ strain (UMY3790). The elp3Δ strain (UMY3790) carrying a pRS315 without insertion serves as control. The transformed yeast cells were spotted on SC-Leu, SC-Leu-Ura and SC-Leu+5-FOA plates, and incubated at 30°C for 2 days. (C) The elp3Δ strain (UMY2843) transformed with the same set of plasmids as in (B) were spotted on SC-Leu, SC-Leu+50 mM hydroxyurea plates, and incubated at 30°C for 2 days.