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Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification

Figure 3

Increased levels of tRNA^Lys_s²UUU, tRNA^Gln_s²UUG, and tRNA^Glu_s²UUC bypass the phenotypes of asf1Δ elp3Δ double mutants.

(A) The asf1Δ elp3Δ strain (UMY3805) was transformed with pRS315-ELP3, pRS425-tK-tQ-tE or pRS425. Transformants were streaked on SC-Leu plates and incubated at 30°C or 37°C for 2 days. (B) Ten fold dilutions of elp3Δ (UMY2843), asf1Δ (UMY3800) and asf1Δ elp3Δ (UMY3805) strains carrying either pRS425-tK-tQ-tE or pRS425 were spotted on SC-Leu and SC-Leu+50 mM HU plates, and incubated 4 days at 30°C. The elp3Δ (UMY2843) transformed with pRS315-ELP3 was used as control. Abbreviations for the tRNA genes encoding tRNA^Lys_UUU, tRNA^Gln_UUG, and tRNA^Glu_UUC are tK, tQ and tE, respectively.

doi: https://doi.org/10.1371/journal.pgen.1002258.g003