Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification
Figure 2
Elevated levels of tRNALyss2UUU, tRNAGlns2UUG, and tRNAGlus2UUC suppress the HU sensitivity induced by Elongator mutants.
(A) The elp3Δ strain (UMY2843) carrying plasmids pRS315-ELP3, pRS425-tK-tQ-tE or pRS425 were 10-fold diluted, spotted on SC-Leu and SC-Leu+50 mM HU plates, and incubated at 30°C for 2 days. (B) The wild type (W303-1A), elp1Δ (UMY3783), elp2Δ (UMY3784), elp4Δ (UMY3785), elp5Δ (UMY3786) and elp6Δ (UMY3787) strains transformed with plasmids pRS425-tK-tQ-tE or pRS425 were assayed as described in (A). Abbreviations for the tRNA genes encoding tRNALysUUU, tRNAGlnUUG, and tRNAGluUUC are tK, tQ and tE, respectively.