Elongator Complex Influences Telomeric Gene Silencing and DNA Damage Response by Its Role in Wobble Uridine tRNA Modification
Figure 1
Increased levels of tRNALyss2UUU, tRNAGlns2UUG, and tRNAGlus2UUC suppress the telomeric silencing defect of Elongator mutants.
(A) The wild type (UMY2584) and elp3Δ (UMY3790) strains were 10-fold diluted, spotted on SC, SC-Ura and SC+5-FOA plates, and incubated at 30°C for 2 days. (B) The elp3Δ strain (UMY3790) with plasmids, pRS315-ELP3, pRS425-tK-tQ-tE or pRS425, were 10-fold diluted, spotted on SC-Leu, SC-Leu-Ura and SC-Leu+5-FOA plates, and incubated at 30°C for 2 days. (C) The wild type (UMY2584), elp1Δ (UMY3788), elp2Δ (UMY3789), elp4Δ (UMY3791), elp5Δ (UMY3792) and elp6Δ (UMY3793) with plasmids pRS425-tK-tQ-tE or pRS425 were treated as described in (B). Abbreviations for the tRNA genes encoding ,
and
are tK, tQ and tE, respectively.