A Quantitative Systems Approach Reveals Dynamic Control of tRNA Modifications during Cellular Stress
Figure 2
Hierarchical cluster analysis of toxicant-induced changes in tRNA modification spectra in wild-type yeast exposed to concentrations of MMS, H2O2, NaOCl, and NaAsO2 producing 20%, 50%, and 80% cytotoxicity.
LC-MS/MS quantification of ribonucleosides was performed as described in Materials and Methods and data expressed as fold-change relative to unexposed controls (Table S2), with hierarchical cluster analysis performed on mean-centered data. The top-left color bar indicates the range of fold-change values.