Pten Dose Dictates Cancer Progression in the Prostate
Figure 4
Ptenpc2 Mice Develop Invasive CaP
(A) Histopathology analysis of wild-type, Ptenpc1, and Ptenpc2 mice prior to tumor onset. H&E-stained AP (top) shows the difference in both the morphology and proliferative rates of the prostatic epithelium in these two models. IHC staining with anti-Pten antibody (bottom) was carried out on wild-type, Ptenpc1, and Ptenpc2 mice. In wild-type mice, strong cytoplasmic staining was observed in epithelial cells (arrowheads). In Ptenpc1 mice, staining was generally weak, whereas in Ptenpc2 mice, staining was completely absent in the prostatic epithelium. Original magnification, 400×.
(B) MRI (top) shows massive prostate tumor (surrounded by dashed line) in Ptenpc2 mice (at 6 mo) and no detectable difference between the prostates of Ptenpc1 or Pten+/+ mice (at 12 mo; arrowheads). Bladder (Bl) and seminal vesicles (SV) are indicated. Macroscopic view (second from top) of the same animals confirms massive enlargement of both APs in Ptenpc2 and reveals the slightly enlarged AP of Ptenpc1 mice (encircled). H&E staining (bottom) of the prostate from Ptenpc1 mice was characterized by multiple foci of PIN lesions and by the presence of prostatic adenocarcinoma. These lesions contained well-differentiated neoplastic cells and showed focal areas of invasion (arrowheads). H&E stainings of prostates from Ptenpc2 mice showed diffuse, invasive CaP with large, undifferentiated tumor cells growing into stromal areas.