Fig. 4

Analysis of glomerular hypertrophy and mesangial expansion. A Representative periodic acid-Schiff (PAS) staining illustrating the morphological evaluation of glomeruli at 14–15 and 18–20 weeks of age. B Evaluation of glomerular tuft area. C Progression of glomerular tuft area at 18–20 weeks of age compared to 14–15 weeks. D Fractioned mesangial expansion. E Progression of fractioned mesangial expansion at 18–20 weeks of age compared to 14–15 weeks. (*p < 0.05). Scale bars represent 20 µm in A. Error bars represent mean ± SEM; n = 5–6 animals