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Fusarium head blight (FHB), caused by the Fusarium species complex, significantly endangers wheat yield and safety. Accurate and timely assessment of FHB epidemic level in the field is crucial for effective disea...
Pepper is an economically important crop. Owing to its recalcitrance to genetic transformation, virus-induced gene silencing (VIGS) is currently the major technique available for validating gene function in pe...
Flavescence dorée (FD) is one of the most damaging grapevine diseases in Europe, caused by the quarantine-listed Grapevine flavescence dorée phytoplasma (FDp). Given the absence of resistant cultivars and curativ...
The photosynthetic pigments – chlorophyll a (Chl a), chlorophyll b (Chl b), and carotenoids (Car) – in juvenile ginkgo leaves are crucial for growth monitoring as they reflect physiological status and directly...
The tea industry plays a vital role in China’s green economy. Tea trees (Melaleuca alternifolia) are susceptible to numerous diseases and pest threats, making timely pathogen detection and precise pest identifica...
Arabidopsis thaliana is the leading model plant used to study plant-pathogen interactions. High-throughput phenotyping allows for the simultaneous study of many plants with high-frequency image acquisition. Never...
In recent years, non-destructive and non-invasive methods for 3D plant reconstruction have gained increasing importance in plant phenotyping. Morphological traits reflect the physiological status of a plant an...
The generation of a large amount of ground truth data is an essential bottleneck for the application of deep learning-based approaches to plant image analysis. In particular, the generation of accurately label...
The use of Illumina sequencing technologies has enabled the identification and removal of mutations in various plant species. However, the Illumina sequencing method requires a considerable amount of data to e...
Agriculture plays a pivotal role in global food security and socio-economic stability, yet crop productivity remains threatened by plant diseases that incur substantial economic losses. Pomegranate is an impor...
Accurate quantification of RNA isoforms is critical for understanding gene regulation. However, conventional reverse transcription-quantitative real-time PCR (RT-qPCR) has limited sensitivity for low-abundance...
Paeonia ostii, an economically important oil-producing peony cultivar, faces challenges in large-scale cultivation due to low propagation rates and long cultivation cycles. This study aimed to optimize tissue cul...
In rice pest management, accurate pest detection is critical for intelligent agricultural systems, yet challenges like limited dataset availability, pest occlusion, and insufficient small object detection accu...
Protein detection on large somatic chromosomes typically includes paraformaldehyde fixation and squashing of enzymatically softened root tips in a buffer. It often suffers from chromosome clumping, poor chromo...
Integrating genotype-by-Environment (GxE) interactions into genomic prediction models has been demonstrated to enhance the accuracy of predictions for crops exposed to unfavourable environmental conditions. Ho...
Tomato early blight, caused by Alternaria solani, poses a significant threat to crop yields. Existing detection methods often struggle to accurately identify small or multi-scale lesions, particularly in early st...
Wheat is significantly impacted by fungal diseases, which result in severe economic losses. These diseases result from pathogenic spores invading wheat. Rapid and accurate detection of these spores is essentia...
Spikelet number, a core phenotypic parameter for wheat yield composition, requires precise estimation through accurate spike contour extraction and differentiation between grain surfaces and spikelet surfaces....
is an important species for establishing pulpwood plantations due to its high application value in engineered wood products. However, the lack of a well-established in vitro regeneration system has severely co...
Fusarium Head Blight (FHB) is a destructive fungal disease affecting wheat and barley, leading to significant yield losses and reduced grain quality. Susceptibility to FHB is influenced by genetic factors, env...
In major soybean-growing regions worldwide, vertical (three-dimensional) planting systems are widely adopted. Achieving precise phenotyping of individual soybean plants is crucial for breeding shade-tolerant c...
Disease incidence is a key factor contributing to reduced crop yield. Thus, early identification of crop diseases is crucial for minimizing the effects of disease incidence and maximizing crop yield. Therefore...
Quantitative understanding of plant carbon (C) metabolism by 13CO2/12CO2-labelling studies requires absence (or knowledge) of C-isotopic contamination artefacts during tracer application and sample processing. Su...
Plant growth prediction assists physiologists and botanists in analyzing future development trends, thereby shortening experimental cycles and reducing costs. Traditional growth prediction methods mainly focus...
To overcome the risk of cellular damage and RNA degradation caused by high temperatures and cellular damage induced by laser capture microdissection (LCM) during plant single cell or small cell cluster isolati...
Rice diseases pose a severe threat to global food security, while traditional detection methods suffer from low efficiency and dependence on manual expertise. To address the challenges of insufficient feature ...
Atriplex canescens, a halophytic model plant exhibiting remarkable tolerance to abiotic stress, possesses valuable genetic resources for crop improvement. However, the absence of a reliable and stable transformat...
Genotype-by-sequencing (GBS) is a cost-effective method for large-scale genotyping, widely used across various species, particularly those with large genomes. A critical aspect of GBS lies in the selection of ...
Deep learning demonstrates strong generalisation capabilities, driving substantial progress in plant disease recognition systems. However, current methods are predominantly optimised for offline implementation...
Herbs have historically been central to medicinal practices, representing one of the earliest forms of therapeutic intervention. While synthetic drugs are often highly effective in treating acute conditions, t...
Mitochondria are central to plant growth, development, and stress resilience. Despite their importance, mitochondrial research in desiccation-tolerant mosses remains underexplored. To unravel the stress resist...
Apple Marssonina blotch (AMB) is a major disease causing pre-mature defoliation. The occurrence of AMB will lead to serious production decline and economic losses. The precise identification of AMB outbreaks and ...
Improving peach cultivars with superior traits is a primary objective of breeding initiatives. In this study, we aimed to elucidate the genetic basis of key agronomic and fruit-related traits using a reproduci...
Wheat dwarf virus (WDV) is a destructive cereal virus causing significant yield losses in wheat and barley. It is transmitted by the leafhopper Psammotettix alienus and can persist in wild grasses between growing...
Xylophilus ampelinus is a plant pathogenic bacterium that causes bacterial blight in grapevines, which can lead to severe yield losses and economic damage. Owing to its fastidious growth on culture media, detecti...
Existing methods for fluoride (F-) determination in plant material require expensive equipment and specialized reagents. This study aimed to develop a simple and cost-effective method for fluoride analysis in pla...
Modern plant breeding strategies rely on the intensive use of advanced genomic tools to expedite the development of improved crop varieties. Genomic DNA extraction from crop seeds eliminates the need to grow p...
Spray-induced gene silencing (SIGS) is a promising strategy for controlling plant diseases caused by pests, fungi, and viruses. The method involves spraying on plant surfaces double-stranded RNAs (dsRNAs) that...
Stomatal morphology plays a critical role in regulating plant gas exchange influencing water use efficiency and ecological adaptability. While traditional methods for analyzing stomatal traits rely on labor-in...
Agricultural systems are under extreme pressure to meet the global food demand, hence necessitating faster crop improvement. Rapid evaluation of the crops using novel imaging technologies coupled with robust i...
Soil compaction is defined as the reduction of air-filled pore space affecting soil density, water conductivity and nutrient availability. These conditions negatively influence root morphology, root developmen...
Fungal diseases are among the most significant threats to global crop production, often leading to substantial yield losses. Early detection of crop infection by fungus is the very first step to deploying a ti...
Tomato brown rugose fruit virus (TBRFV; Tobamovirus fructirugosum) is a highly virulent tobamovirus that has emerged as a major global threat to tomato and pepper crops over the past decade. Early and ultra-sensi...
The original article was published in Plant Methods 2023 19:139
Global warming is currently occurring at a rapid rate and is having a particularly severe impact on plants, which, as sessile organisms, have a limited ability to escape high temperatures. This requires a bett...
Citrus leaf diseases significantly affect production efficiency and fruit quality in the citrus industry. To effectively identify and classify citrus leaf diseases, this study proposed a classification approac...
Transgenic plants are essential for both basic and applied plant biology. Recently, fluorescent and colorimetric markers were developed to enable nondestructive identification of transformed seeds and accelera...
The diverse visual features of tobacco leaves during various curing stages are influenced by multiple factors such as the origin of the tobacco and the environment of the curing room, making precise identifica...
Developing disease-resistant crops is a critical strategy for reducing chemical treatments and mitigating plant disease outbreaks, particularly amid global environmental changes. Fusarium head blight (FHB), ca...
Climate change is gradually increasing demand for resilient, nutritious crops like finger millet or ragi. Ensuring food security requires researchers to develop improved and adapted cultivars rapidly. Modern t...
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Plant Methods