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Developing nerve cell clusters in the subesophageal ganglion of a stage 3 Pseudopallene sea spider. The image shows a virtual transverse section moving from back to front through the ganglion, created using a 3D reconstruction program on confocal laser-scanning microscopy data. The sample is stained and immunolabeled to highlight acetylated tubulin in red (marking parts of the cytoskeleton), a specific phospho-histone in yellow (marking dividing cells), and Hoechst stain H33342 in blue (marking cell nuclei). One histone-labeled cell visible at around 19s in the left front stream is in prophase, an early stage of cell division. On the right side, the image sequence enters the more sideward (antero-lateral) palpal cell cluster. Several flattened glial cell nuclei are visible in the nerve sheath. The front-upper (antero-dorsal) part of the ganglion shows slight damage.
 


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