Microorganisms can be beneficial or harmful. They are classified into five major groups - bacteria, viruses, protozoa, algae, and fungi. Bacteria are single-celled prokaryotes that can be beneficial, parasitic or saprophytic. Viruses are not considered living but can only replicate inside host cells. Protozoa are single-celled organisms that can cause diseases. Fungi include multicellular and unicellular organisms that reproduce via spores. Microorganisms are used in food production, medicine development, pollution reduction and nitrogen fixation in plants. However, they can also cause diseases and food poisoning in humans, animals and plants.