Thiamine-repressible expression vectors pREP and pRIP for fission yeast
- PMID: 8422996
- DOI: 10.1016/0378-1119(93)90551-d
Thiamine-repressible expression vectors pREP and pRIP for fission yeast
Abstract
The promoter and polyadenylation signal of the thiamine-repressible gene nmt1 of Schizosaccharomyces pombe have been used to construct the pREP extrachromosomally replicating plasmids and the pRIP integrative expression plasmids. These plasmids permit thiamine-mediated control of transcription to be applied to cloned genes.
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