Skip to main page content
U.S. flag
See More

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Sep 15;90(18):8429-33.
doi: 10.1073/pnas.90.18.8429.

Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction

O Silvennoinen  1 , B A Witthuhn, F W Quelle, J L Cleveland, T Yi, J N Ihle
Affiliations

Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction

O Silvennoinen et al. Proc Natl Acad Sci U S A. .

Abstract

Interleukin 3 (IL-3) regulates the proliferation and differentiation of hematopoietic cells. Although the IL-3 receptor chains lack kinase catalytic domains, IL-3 induces tyrosine phosphorylation of cellular proteins. To investigate the potential role of the JAK family of protein-tyrosine kinases in IL-3 signal transduction, we have obtained full-length cDNA clones for murine Jak1 and Jak2 protein-tyrosine kinases and prepared antiserum against the predicted proteins. Using antisera against Jak2, we demonstrate that IL-3 stimulation results in the rapid and specific tyrosine phosphorylation of Jak2 and activates its in vitro kinase activity.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1991 Apr;11(4):1820-8 - PubMed
    1. Science. 1988 Jul 1;241(4861):42-52 - PubMed
    1. Methods Enzymol. 1991;200:533-46 - PubMed
    1. Chem Immunol. 1992;51:65-106 - PubMed
    1. Annu Rev Immunol. 1992;10:295-331 - PubMed

Publication types

MeSH terms

Associated data