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Comparative Study
. 1994 Aug;126(4):853-62.
doi: 10.1083/jcb.126.4.853.

Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus

X Shan  1 , Z Xue, T Mélèse
Affiliations
Comparative Study

Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus

X Shan et al. J Cell Biol. 1994 Aug.

Abstract

We have identified a gene (NPI46) encoding a new prolyl cis-trans isomerase within the nucleolus of the yeast Saccharomyces cerevisiae. The protein encoded by NPI46 was originally found by us in a search for proteins that recognize nuclear localization sequences (NLSs) in vitro. Thus, NPI46 binds to affinity columns that contain a wild-type histone H2B NLS but not a mutant H2B NLS that is incompetent for nuclear localization in vivo. NPI46 has two domains, a highly charged NH2 terminus similar to two other mammalian nucleolar proteins, nucleolin and Nopp140, and a COOH terminus with 45% homology to a family of mammalian and yeast proline isomerases. NPI46 is capable of catalyzing the prolyl cis-trans isomerization of two small synthetic peptides, succinyl-Ala-Leu-Pro-Phe-p-nitroanilide and succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, as measured by a chymotrypsin-coupled spectrophotometric assay. By indirect immunofluorescence we have shown that NPI46 is a nucleolar protein. NPI46 is not essential for cell viability.

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References

    1. Gene. 1988 Jul 15;67(1):31-40 - PubMed
    1. Gene. 1991 Dec 1;108(1):73-80 - PubMed
    1. Science. 1991 Aug 23;253(5022):905-9 - PubMed
    1. J Cell Sci. 1993 Jul;105 ( Pt 3):799-806 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 - PubMed

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