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. 1994 Jul 5;91(14):6288-92.
doi: 10.1073/pnas.91.14.6288.

Double-stranded RNA-dependent protein kinase activates transcription factor NF-kappa B by phosphorylating I kappa B

A Kumar  1 , J Haque, J Lacoste, J Hiscott, B R Williams
Affiliations

Double-stranded RNA-dependent protein kinase activates transcription factor NF-kappa B by phosphorylating I kappa B

A Kumar et al. Proc Natl Acad Sci U S A. .

Abstract

The induction of interferon (IFN) genes by viruses or double-stranded RNA (dsRNA) requires the assembly of a complex set of transcription factors on responsive DNA elements of IFN gene promoters. One of the factors necessary for regulating IFN-beta gene transcription is nuclear factor NF-kappa B, the activation of which is triggered by dsRNA. It has previously been suggested that the dsRNA-activated p68 protein kinase (PKR) may act as an inducer-receptor, transducing the signal from dsRNA to NF-kappa B through phosphorylation of the inhibitor I kappa B. We present direct evidence that PKR can phosphorylate I kappa B-alpha (MAD-3) and activate NF-kappa B DNA binding activity in vitro. We further show that dsRNA induces an unusual phosphorylated form of I kappa B-alpha. The expression of a transdominant mutant PKR is able to perturb the dsRNA-mediated signaling pathway in vivo, suggesting a role for this kinase in IFN-beta gene induction.

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References

    1. Cell. 1986 Jan 31;44(2):261-72 - PubMed
    1. J Virol. 1993 Dec;67(12):7695-700 - PubMed
    1. Science. 1988 Apr 8;240(4849):210-3 - PubMed
    1. J Gen Virol. 1988 Jul;69 ( Pt 7):1637-45 - PubMed
    1. EMBO J. 1988 Mar;7(3):751-9 - PubMed

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