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. 1983 Mar 11;11(5):1475-89.
doi: 10.1093/nar/11.5.1475.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

J D Dignam, R M Lebovitz, R G Roeder
Free PMC article

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

J D Dignam et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

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