Selective 40S Footprinting Reveals Cap-Tethered Ribosome Scanning in Human Cells
- PMID: 32589966
- DOI: 10.1016/j.molcel.2020.06.005
Selective 40S Footprinting Reveals Cap-Tethered Ribosome Scanning in Human Cells
Abstract
Translation regulation occurs largely during the initiation phase. Here, we develop selective 40S footprinting to visualize initiating 40S ribosomes on endogenous mRNAs in vivo. This reveals the positions on mRNAs where initiation factors join the ribosome to act and where they leave. We discover that in most human cells, most scanning ribosomes remain attached to the 5' cap. Consequently, only one ribosome scans a 5' UTR at a time, and 5' UTR length affects translation efficiency. We discover that eukaryotic initiation factor 3B (eIF3B,) eIF4G1, and eIF4E remain bound to 80S ribosomes as they begin translating, with a decay half-length of ∼12 codons. Hence, ribosomes retain these initiation factors while translating short upstream open reading frames (uORFs), providing an explanation for how ribosomes can reinitiate translation after uORFs in humans. This method will be of use for studying translation initiation mechanisms in vivo.
Keywords: cap-tethering; eukaryotic initiation factor; mRNA cap; reinitiation; ribosome footprinting; scanning; translation initiation; translational regulation.
Copyright © 2020 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of Interests The authors declare no competing interests.
Comment in
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Should I Stay or Should I Go: eIF3 Remains Ribosome Associated and Is Required for Elongation.Mol Cell. 2020 Aug 20;79(4):539-541. doi: 10.1016/j.molcel.2020.07.025. Mol Cell. 2020. PMID: 32822578
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