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. 2015 Mar 12;589(6):744-9.
doi: 10.1016/j.febslet.2015.02.003. Epub 2015 Feb 11.

Localization of Atg3 to autophagy-related membranes and its enhancement by the Atg8-family interacting motif to promote expansion of the membranes

Machiko Sakoh-Nakatogawa  1 , Hiromi Kirisako  2 , Hitoshi Nakatogawa  3 , Yoshinori Ohsumi  4
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Free article

Localization of Atg3 to autophagy-related membranes and its enhancement by the Atg8-family interacting motif to promote expansion of the membranes

Machiko Sakoh-Nakatogawa et al. FEBS Lett. .
Free article

Abstract

The E2 enzyme Atg3 conjugates the ubiquitin-like protein Atg8 to phosphatidylethanolamine (PE) to drive autophagosome formation in Saccharomyces cerevisiae. In this study, we show that Atg3 localizes to the pre-autophagosomal structure (PAS) and the isolation membrane (IM), providing crucial evidence that Atg8-PE conjugates are produced on these structures. We also find that mutations in the Atg8-family interacting motif (AIM) of Atg3 significantly impairs the PAS/IM localization of Atg3, resulting in inefficient IM expansion. It is suggested that the AIM-mediated PAS/IM localization of Atg3 facilitates membrane expansion in these structures probably by ensuring active production of Atg8-PE on the membranes.

Keywords: Autophagosome; E2 enzyme; Lipidation; Ubiquitin-like protein; Yeast.

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