Skip to main page content
U.S. flag
See More

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Sep;187(4):1058-1074.
doi: 10.1111/j.1469-8137.2010.03346.x. Epub 2010 Jul 1.

Suppression of the AvrBs1-specific hypersensitive response by the YopJ effector homolog AvrBsT from Xanthomonas depends on a SNF1-related kinase

Robert Szczesny  1 , Daniela Büttner  1 , Lucia Escolar  1 , Sebastian Schulze  1 , Anja Seiferth  1 , Ulla Bonas  1
Affiliations
Free article

Suppression of the AvrBs1-specific hypersensitive response by the YopJ effector homolog AvrBsT from Xanthomonas depends on a SNF1-related kinase

Robert Szczesny et al. New Phytol. 2010 Sep.
Free article

Abstract

*Pathogenicity of the Gram-negative plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) depends on a type III secretion system that translocates a cocktail of > 25 type III effector proteins into the plant cell. *In this study, we identified the effector AvrBsT as a suppressor of specific plant defense. AvrBsT belongs to the YopJ/AvrRxv protein family, members of which are predicted to act as proteases and/or acetyltransferases. *AvrBsT suppresses the hypersensitive response (HR) that is elicited by the effector protein AvrBs1 from Xcv in resistant pepper plants. HR suppression occurs inside the plant cell and depends on a conserved predicted catalytic residue of AvrBsT. Yeast two-hybrid based analyses identified plant interaction partners of AvrBs1 and AvrBsT, including a putative regulator of sugar metabolism, SNF1-related kinase 1 (SnRK1), as interactor of AvrBsT. Intriguingly, gene silencing experiments revealed that SnRK1 is required for the induction of the AvrBs1-specific HR. *We therefore speculate that SnRK1 is involved in the AvrBsT-mediated suppression of the AvrBs1-specific HR.

PubMed Disclaimer

References

    1. Abramovitch RB, Martin GB. 2004. Strategies used by bacterial pathogens to suppress plant defenses. Current Opinion in Plant Biology 7: 356-364.
    1. Ade J, DeYoung BJ, Golstein C, Innes RW. 2007. Indirect activation of a plant nucleotide binding site-leucine-rich repeat protein by a bacterial protease. Proceedings of the National Academy of Sciences, USA 104: 2531-2536.
    1. Anand A, Krichevsky A, Schornack S, Lahaye T, Tzfira T, Tang Y, Citovsky V, Mysore KS. 2007. Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plants. Plant Cell 19: 1695-1708.
    1. Astua-Monge G, Minsavage GV, Stall RE, Vallejos CE, Davis MJ, Jones JB. 2000. Xv4-avrXv4: a new gene-for-gene interaction identified between Xanthomonas campestris pv. vesicatoria race T3 and the wild tomato relative Lycopersicon pennellii. Molecular Plant-Microbe Interactions 13: 1346-1355.
    1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. 1996. Current protocols in molecular biology. New York, NY, USA: John Wiley & Sons, Inc.

Publication types

MeSH terms

Substances

LinkOut - more resources