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. 1990 May;87(9):3518-21.
doi: 10.1073/pnas.87.9.3518.

Excitatory amino acid uptake and N-methyl-D-aspartate-mediated secretion in a neural cell line

B H Morimoto  1 , D E Koshland Jr
Affiliations

Excitatory amino acid uptake and N-methyl-D-aspartate-mediated secretion in a neural cell line

B H Morimoto et al. Proc Natl Acad Sci U S A. 1990 May.

Abstract

A functional N-methyl-D-aspartate (NMDA) receptor has been identified on HT-4 cells, a clonal neural cell line, in which glutamate activates the receptor to elicit neurotransmitter secretion. Specific inhibitors of the NMDA receptor block glutamate-mediated secretion, and the characteristics of NMDA-mediated secretion parallel the reported properties of the NMDA receptor. Excitatory amino acid secretion can be elicited by potassium-evoked depolarization and is not the simple reversal of the uptake system. 2-Amino-4-phosphonobutyrate (APB) inhibits depolarization-induced secretion of excitatory amino acids but has no effect on excitatory amino acid uptake, suggesting that the APB binding protein in the brain represents a component involved in the secretion of excitatory amino acids.

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