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. 2009 Mar;37(4):1335-52.
doi: 10.1093/nar/gkn1023. Epub 2009 Jan 16.

Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions

Akiko Noma  1 , Yuriko Sakaguchi, Tsutomu Suzuki
Affiliations

Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions

Akiko Noma et al. Nucleic Acids Res. 2009 Mar.

Abstract

The wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group confers conformational rigidity of mcm(5)s(2)U by largely fixing the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing. We have identified five genes in Saccharomyces cerevisiae, YIL008w (URM1), YHR111w (UBA4), YOR251c (TUM1), YNL119w (NCS2) and YGL211w (NCS6), that are required for 2-thiolation of mcm(5)s(2)U. An in vitro sulfur transfer experiment revealed that Tum1p stimulated the cysteine desulfurase of Nfs1p, and accepted persulfide sulfurs from Nfs1p. URM1 is a ubiquitin-related modifier, and UBA4 is an E1-like enzyme involved in protein urmylation. The carboxy-terminus of Urm1p was activated as an acyl-adenylate (-COAMP), then thiocarboxylated (-COSH) by Uba4p. The activated thiocarboxylate can be utilized in the subsequent reactions for 2-thiouridine formation, mediated by Ncs2p/Ncs6p. We could successfully reconstitute the 2-thiouridine formation in vitro using recombinant proteins. This study revealed that 2-thiouridine formation shares a pathway and chemical reactions with protein urmylation. The sulfur-flow of eukaryotic 2-thiouridine formation is distinct mechanism from the bacterial sulfur-relay system which is based on the persulfide chemistry.

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Figures

Figure 1.
Figure 1.
Chemical structure of 5-methoxycarmonylmethyl-2-thiouridene (mcm5s2U) and secondary structure of tRNAGlu. (A) Chemical structures of mcm5U and mcm5s2U. 2-thiolation of mcm5s2U utilizes cysteine and ATP as substrates. (B) Secondary structure of S. cerevisiae tRNAGlu with modified nucleosides: 5-methoxycarmonylmethyl-2-thiouridine (mcm5s2U), pseudouridine (Ψ), dihydrouridine (D), 5-methylcytidine (m5C) and 5-methyluridine (m5U). Arrows indicate the sites for RNase T1 cleavage needed to produce the anticodon-containing fragment.
Figure 2.
Figure 2.
Mass spectrometric analyses of total nucleosides and purified tRNAsGlu from S. cerevisiae wild-type and mutant cells. (A) LC/MS analyses of total nucleosides from strains of wild-type (WT), ΔYOR251c (TUM1), ΔYHR111w (UBA4), ΔYIL008w (URM1), ΔYNL119w (NCS2) and ΔYGL211w (NCS6). The upper panels show the merged mass chromatograms detecting MH+ (m/z 333) and BH2+ (m/z 201) of mcm5s2U. The lower panels show the mass chromatograms detecting MH+ (m/z 317) and BH2+ (m/z 185) of mcm5U. Arrows in the upper panels indicate the retention time for mcm5s2U. (B) LC/MS fragment analyses of RNase T1-digested tRNAsGlu obtained from wild-type strains: ΔTUM1, ΔUBA4, ΔURM1, ΔNCS2, ΔNCS6 and ΔTRM9. A graph on the top-right represents the mass spectrum for the anticodon-containing fragment (CUmcm5s2UUCACCGp) (Figure 1B) from the wild-type strain. Charge states of multiply charged ions are indicated in parentheses. Other graphs describe the mass chromatograms shown by triply charged ions of anticodon-containing fragments bearing mcm5s2U (m/z 1458.17, red line), mcm5U (m/z 1450.18, black line), ncm5s2U (m/z 1450.67, green line) and ncm5U (m/z 1443.18, blue line). The RNA sequence including the wobble modification is indicated on each graph.
Figure 3.
Figure 3.
Sequence alignments of TUM1, UBA4 and URM1. Each protein is aligned with its homologs. Multiple alignment of each sequence was carried out by Clustal X analysis (82) and displayed using the Genedoc multiple sequence alignment editor. White letters in black boxes represent amino-acid residues identical in all species, while white letters in gray boxes represent residues with ∼80% homology. Black letters in gray boxes represent restudies with ∼60% homology. (A) Sequence alignment of TUM1 with its homologs. Two conserved rhodanese domains are underlined. The conserved C259 is indicated. (B) Sequence alignment of UBA4 with its homologs and E. coli ThiF and MoeB. The boxed regions are the ATP-binding motif (GxGxxG) and the metal-binding motif (CxxC, CxxCG). The conserved C225 and C397 are indicated. The rhodanese-like domain in the C-terminal region is underlined. (C) Sequence alignment of URM1 with its homologs and E. coli ThiS and MoaD. The conserved C-terminal GG motif is boxed.
Figure 4.
Figure 4.
APM Northern analyses of thiolation-status of tRNAs in mutant strains and transformants. (A) APM northern analyses of tRNAGlu in the s2U-deficient strains by introducing a series of mutant plasmids. Total RNA from each strain was resolved by denaturing polyacrylamide-gel electrophoresis in the presence (upper panels) or absence (lower panels) of APM. 2-thiolated tRNAGlu in each strain was detected as a shifted band only in the presence of APM, by northern blotting. Lanes 1 to 12 correspond to wild-tye (1), ΔURM1 (2), ΔURM1 harboring pURM1 (3), ΔURM1 harboring pURM1ΔGG (4), ΔUBA4 (5), ΔUBA4 harboring pUBA4 (6), ΔUBA4 harboring pUBA4 C225S (7), ΔUBA4 harboring pUBA4 C225A (8), ΔUBA4 harboring pUBA4 C397S (9),ΔTUM1 (10), ΔTUM1 harboring pTUM1 (11), ΔTUM1 harboring pTUM1 C259S (12), ΔTUM1 harboring pTUM1 C259A (13), ΔNCS2 (14) and ΔNCS2 harboring pNCS2 (15), respectively. (B) Quantification of the thiolation-status of tRNAGlu by APM/northern analyses. The fraction of the retarded band in the total intensity of the bands was calculated. Data are shown as values ± SD and reflect the average of four independent experiments. (C) APM/northern analyses of tRNAsLys from wild-type, ΔTRM9, ΔELP4, ΔTUM1 and ΔURM1. The right panel represents a mass chromatogram shown by triply charged ions of the anticodon-containing fragments of tRNAGlu isolated from ΔELP4. The unmodified fragment (m/z 1414.18, black line) and the s2U-containing fragment (m/z 1422.16, gray line) are specifically detected.
Figure 5.
Figure 5.
In vitro sulfur transfer from Nfs1p to Tum1p and Uba4p. (A) Sulfur transfer from Nfs1p to Tum1p. The gel was stained with Coomassie brilliant blue (CBB) (upper panel) and [35S] radioactivity was visualized on a phosphor-imaging plate (lower panel). Lane 1, Nfs1p (25 pmol); lane 2, Tum1p (25 pmol); lane 3, Nfs1p (25 pmol) and Tum1p (25 pmol), lanes 4–9, Nfs1p (25 pmol) and Tum1p (2.5, 5, 12.5, 25, 37.5, 50 pmol). (B) Sulfur transfer from Nfs1p to Uba4p. The gel was stained with Coomassie brilliant blue (CBB) (upper panel) and [35S] radioactivity was visualized on a phosphor-imaging plate (lower panel). Lane 1, Nfs1p (25 pmol); lane 2, Uba4p (25 pmol); lane 3, Nfs1p (25 pmol) and Uba4p (25 pmol), lanes 4–9, Nfs1p (25 pmol) and Uba4p (2.5, 5, 12.5, 25, 37.5, 50 pmol).
Figure 6.
Figure 6.
Thiocarboxylation of Urm1p through an acyl-adenylated intermediate. (A) In vitro sulfur transfer from Nfs1p to Urm1p. Recombinant Nfs1p, Tum1p, Urm1p and Uba4p were mixed and incubated with [35S] cysteine in the presence (lane 1) or absence (lane 2) of ATP. The reaction mixture was further incubated with 50 mM DTT (lane 3). The gel was stained with CBB (upper panels) and [35S] radioactivity was visualized on a phosphor-imaging plate (lower panels). The band for each protein is indicated. (B) Whole-mass analysis of the thiocarboxylation of Urm1p. Left panels are mass chromatograms for detecting +9-charged ions of Urm1p with unmodified C-terminus (-COOH, black line), Urm1- with thiocarboxylated C-terminus (-COSH, red line) and Urm1p with acyl-adenylated C-terminus (-COAMP, green line). Right panels are deconvoluted mass spectra for Urm1p detecting unmodified Urm1p (-COOH, 12 083 Da), thiocarboxylated Urm1p (-COSH, 12099 Da) and acyl-adenylated Urm1p (-COAMP, 12 413 Da). Top panels show whole-mass analysis of purified Urm1p used in this study. In vitro thiocarboxylation of Urm1p by Uba4p was carried out in the absence (middle panels) or presence (bottom panels) of ATP. (C) Mass spectrum of Urm1p with an acyl-adenylated C-terminus (-COAMP). A series of multiply charged ions are detected. The parent ion (m/z 1380.022, +9) for collision-induced dissociation (CID) analysis is indicated. (D) CID spectrum of Urm1p-COAMP. The product ion (m/z 348.095) for AMP originated from Urm1p-COAMP is shown.
Figure 7.
Figure 7.
Reconstitution of 2-thiouridine formation in vitro using recombinant proteins. Thiouridine formation was detected by retardation of the tRNA band on the gel in the presence (lanes 1–4) or absence (lanes 5–8) of APM. 2-thiouridine formation of [32P]-labeled tRNALys2 was carried out with recombinant proteins (Nfs1p, Tum1p, Uba4p and Urm1p) in the presence (lanes 1and 5) or absence (lanes 2 and 6) of IgG beads retaining the Ncs6p, or in the presence of IgG beads treated with wild-type cell lysate (lanes 4 and 8) as a negative control. The reaction was also performed only in the presence of IgG beads retaining the Ncs6p without recombinant proteins (lanes 3 and 7).
Figure 8.
Figure 8.
Comparison of cellular sulfur trafficking related to thiouridine formation of tRNA anticodon in S. cerevisiae and E. coli. (A) Proposed sulfur flow system for 2-thiouridine formation of mcm5s2U. Nfs1p accepts sulfur from Cys to form a persulfide using PLP as a cofactor. The persulfide sulfur is mainly transferred to the RLD2 (Cys259) of Tum1p, and partially to the RLD (Cys397) of Uba4p. Uba4p, as an E1-like enzyme in the ubiquitin-related pathway, activates the C-terminus of Urm1p to form the acyl-adenylated intermediate, then transfers the persulfide sulfur from the RLD to form thiocarboxylated Urm1p (Urm1p-COSH) by releasing AMP. Urm1p-COSH is a substrate of 2-thiouridine formation catalyzed by Ncs2p and Ncs6p. For protein urmylation, Urm1p-COSH is conjugated with Uba4p, then the putative E3 enzyme transfers Urm1p to target proteins such as Ahp1p. (B) Sulfur-relay system by Tus-proteins in E. coli. IscS accepts sulfur from Cys to form a persulfide using PLP as a cofactor. TusA interacts with IscS to stimulate its desulfurase activity, and accepts the persulfide sulfur. The sulfur is transferred to TusD in the TusBCD complex. It is then transferred to TusE. TusE interacts with MnmA to transfer the sulfur. MnmA recognizes the tRNA and activates the wobble uridine by forming an adenylated intermediate, then the persulfide sulfur attacks this position to release the AMP resulting in the synthesis of the 2-thiouridine of mnm5s2U.
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