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. 2007 Jul;45(7):2289-92.
doi: 10.1128/JCM.00349-07. Epub 2007 May 23.

RTX toxin actin cross-linking activity in clinical and environmental isolates of Vibrio cholerae

Christina L Cordero  1 , Shanmuga Sozhamannan, Karla J Fullner Satchell
Affiliations

RTX toxin actin cross-linking activity in clinical and environmental isolates of Vibrio cholerae

Christina L Cordero et al. J Clin Microbiol. 2007 Jul.

Abstract

Vibrio cholerae strains from diverse O-antigen groups were evaluated for RTX toxin actin cross-linking activity. This study demonstrates that the actin cross-linking domain sequence is present within rtxA in the majority of clinical and environmental isolates tested, and the RTX toxin produced by these strains catalyzes the covalent cross-linking of cellular actin.

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Figures

FIG. 1.
FIG. 1.
The rtxA gene and ACD sequence are present in non-O1, non-O139 serogroups of V. cholerae. The sequences for rtxA and the ACD were amplified by colony PCR for each bacterial strain. The resulting PCR products were separated on a 1% agarose gel, and rtxA and the ACD were detected by ethidium bromide staining. A representative sample of strains is shown, and the complete list of results is displayed in Table 1. Vv, V. vulnificus strain MO6-24/O.
FIG. 2.
FIG. 2.
V. cholerae non-O1, non-O139 strains cause actin cross-linking in vivo. HEp-2 cells were incubated with phosphate-buffered saline (PBS) or the V. cholerae and V. vulnificus strains listed in Table 1. Cell lysates were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and actin cross-linking was detected by immunoblotting with an anti-actin antibody. A representative sample of strains is shown, and the complete list of results is displayed in Table 1. Vv, V. vulnificus strain MO6-24/O. The values on the left are molecular sizes in kilodaltons.
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References

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