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Clinical Trial
. 2006 Sep;19(9):759-68.
doi: 10.1111/j.1432-2277.2006.00351.x.

Urinary cytotoxic molecular markers for a noninvasive diagnosis in acute renal transplant rejection

Maria Yannaraki  1 , Jean-Michel Rebibou, Didier Ducloux, Philippe Saas, Anne Duperrier, Sophie Felix, Gérard Rifle, Jean-Marc Chalopin, Patrick Hervé, Pierre Tiberghien, Christophe Ferrand
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Free article
Clinical Trial

Urinary cytotoxic molecular markers for a noninvasive diagnosis in acute renal transplant rejection

Maria Yannaraki et al. Transpl Int. 2006 Sep.
Free article

Abstract

Perforin (P), Granzyme B (GB) and Fas-Ligand (FAS-L) are cytotoxic molecules involved in acute rejection (AR) after renal transplantation. A noninvasive diagnostic test to monitor AR and other complications could improve clinical management. We investigated the predictive and diagnostic interest of target mRNA measurements, with a quantitative PCR assay, in AR, as well as in other clinical complications recurrent in kidney transplantation. One hundred and sixty-two urine specimens from 37 allograft recipients were investigated. Clinical settings were AR, urinary tract infection (UTI), cytomegalovirus infection (CMVi) or disease (CMVd), chronic allograft nephropathy (CAN), delayed graft function (DGF) and stable graft course (controls). In the case of AR, mRNA levels of all three molecules were significantly higher than in recipients not showing any clinically evident signs of complication. Indeed, it was observed that expression levels of P, GB and Fas-L mRNA also increase in other clinical situations such as UTI, CMV and DGF. Finally, kinetic studies in three patients with AR revealed that increased P, GB and Fas-L mRNA levels could precede or were concomitant with increased serum creatinin levels. P, GB and Fas-L gene expression in urine specimens were upregulated in AR episodes but also in UTI, CMV infection and DGF. Therefore, this technique would appear to be of limited clinical value as a noninvasive method of diagnosing AR.

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