Skip to main page content
U.S. flag
See More

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Oct 6;348(4):1358-66.
doi: 10.1016/j.bbrc.2006.07.195. Epub 2006 Aug 7.

eIF5A binds to translational machinery components and affects translation in yeast

Cleslei F Zanelli  1 , Ana L C Maragno, Ana P B Gregio, Suzanne Komili, José R Pandolfi, Carlos A Mestriner, Wilton R Lustri, Sandro R Valentini
Affiliations

eIF5A binds to translational machinery components and affects translation in yeast

Cleslei F Zanelli et al. Biochem Biophys Res Commun. .

Abstract

The putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources