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. 2006 Sep 15;313(5793):1642-5.
doi: 10.1126/science.1127344. Epub 2006 Aug 10.

Imaging intracellular fluorescent proteins at nanometer resolution

Eric Betzig  1 , George H Patterson, Rachid Sougrat, O Wolf Lindwasser, Scott Olenych, Juan S Bonifacino, Michael W Davidson, Jennifer Lippincott-Schwartz, Harald F Hess
Affiliations

Imaging intracellular fluorescent proteins at nanometer resolution

Eric Betzig et al. Science. .

Abstract

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.

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  • The limits of light.
    Schuldt A. Schuldt A. Nat Rev Mol Cell Biol. 2010 Oct;11(10):678. doi: 10.1038/nrm2989. Nat Rev Mol Cell Biol. 2010. PMID: 20861874 No abstract available.

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