Standing up is an important and common daily activity. It is essential for independence and a prerequisite for walking. Many elderly and many subjects with impairments have problems with transition from sitting to standing. The aim of the present study was to determine whether there was any difference between the characteristics of standing up in trans-femoral amputees and healthy subjects. Five young trans-femoral amputees and five healthy subjects were included in the study. They were asked to stand up. The body motion was recorded using an Optotrak contactless optical system. The force and moment vectors exerted on the seat were recorded by a JR3 six-axis robot wrist sensor. The force under the feet was recorded by two AMTI force plates. The trans-femoral amputees were found to stand up more slowly than the healthy subjects. The angles of the hip, knee, and ankle joints on the amputated side were different from the angles on the healthy side or in the healthy subjects. There was also a great difference in loading between the healthy and the prosthetic foot. It can be concluded that there are differences in standing up between the trans-femoral amputees and the healthy subjects. These differences may indicate a reason for problems many elderly trans-femoral amputees face when standing up.