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. 2004 Feb 27;279(9):7925-33.
doi: 10.1074/jbc.M307807200. Epub 2003 Dec 10.

Differential signaling to apoptotic and necrotic cell death by Fas-associated death domain protein FADD

Tom Vanden Berghe  1 , Geert van Loo, Xavier Saelens, Maria Van Gurp, Greet Brouckaert, Michael Kalai, Wim Declercq, Peter Vandenabeele
Affiliations
Free article

Differential signaling to apoptotic and necrotic cell death by Fas-associated death domain protein FADD

Tom Vanden Berghe et al. J Biol Chem. .
Free article

Abstract

Two general pathways for cell death have been defined, apoptosis and necrosis. Previous studies in Jurkat cells have demonstrated that the Fas-associated death domain (FADD) is required for Fas-mediated signaling to apoptosis and necrosis. Here we developed L929rTA cell lines that allow Tet-on inducible expression and FK506-binding protein (FKBP)-mediated dimerization of FADD, FADD-death effector domain (FADD-DED), or FADD-death domain (FADD-DD). We show that expression and dimerization of FADD leads to necrosis. However, pretreatment of the cells with the Hsp90 inhibitor geldanamycin, which leads to proteasome-mediated degradation of receptor interacting protein 1 (RIP1), reverts FKBP-FADD-induced necrosis to apoptosis. Expression and dimerization of FADD-DD mediates necrotic cell death. We found that FADD-DD is able to bind RIP1, another protein necessary for Fas-mediated necrosis. Expression and dimerization of FADD-DED initiates apoptosis. Remarkably, in the presence of caspase inhibitors, FADD-DED mediates necrotic cell death. Coimmunoprecipitation studies revealed that FADD-DED in the absence procaspase-8 C/A is also capable of recruiting RIP1. However, when procaspase-8 C/A and RIP1 are expressed simultaneously, FADD-DED preferentially recruits procaspase-8 C/A.

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