Skip to main page content
U.S. flag
See More

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Oct 3;278(40):38117-20.
doi: 10.1074/jbc.C300330200. Epub 2003 Aug 20.

Phosphorylation of serine 13 is required for the proper function of the Hsp90 co-chaperone, Cdc37

Jieya Shao  1 , Thomas Prince, Steven D Hartson, Robert L Matts
Affiliations
Free article

Phosphorylation of serine 13 is required for the proper function of the Hsp90 co-chaperone, Cdc37

Jieya Shao et al. J Biol Chem. .
Free article

Abstract

The Hsp90 co-chaperone Cdc37 provides an essential function for the biogenesis and support of numerous protein kinases. In this report, we demonstrate that mammalian Cdc37 is phosphorylated on Ser13 in situ in rabbit reticulocyte lysate and in cultured K562 cells and that casein kinase II is capable of quantitatively phosphorylating recombinant Cdc37 at this site. Mutation of Ser13 to either Ala or Glu compromises the recruitment of Cdc37 to Hsp90-kinase complexes but has only modest effects on its basal (client-free) binding to Hsp90. Furthermore, Cdc37 containing the complementing Ser to Glu mutation showed altered interactions with Hsp90-kinase complexes consistent with compromised Cdc37 modulation of the Hsp90 ATP-driven reaction cycle. Thus, the data indicate that phosphorylation of Cdc37 on Ser13 is critical for its ability to coordinate Hsp90 nucleotide-mediated conformational switching and kinase binding.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources