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. 2002 Mar;13(3):847-53.
doi: 10.1091/mbc.01-12-0588.

Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae

Kai Stefan Dimmer  1 , Stefan Fritz, Florian Fuchs, Marlies Messerschmitt, Nadja Weinbach, Walter Neupert, Benedikt Westermann
Affiliations

Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae

Kai Stefan Dimmer et al. Mol Biol Cell. 2002 Mar.

Abstract

The understanding of the processes underlying organellar function and inheritance requires the identification and characterization of the molecular components involved. We pursued a genomic approach to define the complements of genes required for respiratory growth and inheritance of mitochondria with normal morphology in yeast. With the systematic screening of a deletion mutant library covering the nonessential genes of Saccharomyces cerevisiae the numbers of genes known to be required for respiratory function and establishment of wild-type-like mitochondrial structure have been more than doubled. In addition to the identification of novel components, the systematic screen revealed unprecedented mitochondrial phenotypes that have never been observed by conventional screens. These data provide a comprehensive picture of the cellular processes and molecular components required for mitochondrial function and structure in a simple eukaryotic cell.

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Figures

Figure 1
Figure 1
Distribution of functional classes of known and newly identified yeast pet genes using criteria from the Yeast Proteome Database (Costanzo et al., 2001). Numbers indicate the number of pet genes falling into each functional class. An annotated list of all identified pet genes catalogued according to their cellular function can be found in supplemental Table 3. mt, mitochondrial.
Figure 2
Figure 2
Mitochondrial morphology of newly identified mdm mutants. Strains expressing mitochondria-targeted GFP were grown in glucose-containing YPD medium at 30°C to logarithmic growth phase and subjected to fluorescence microscopy. Left, mitochondrial morphology of representative cells; right, overlay with the corresponding phase contrast image. WT, wild-type. Bar, 5 μm.
See this image and copyright information in PMC

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