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Comparative Study
. 2001 May;45(5):1568-71.
doi: 10.1128/AAC.45.5.1568-1571.2001.

In vitro activity of clarithromycin against intracellular Helicobacter pylori

R Piccolomini  1 , G Di Bonaventura, C Picciani, F Laterza, J Vecchiet, M Neri
Affiliations
Comparative Study

In vitro activity of clarithromycin against intracellular Helicobacter pylori

R Piccolomini et al. Antimicrob Agents Chemother. 2001 May.

Abstract

The in vitro intracellular effect of clarithromycin, amoxicillin, metronidazole, lansoprazole, and rifabutin, tested at concentrations corresponding to one times the MIC, two times the MIC, and four times the MIC, was evaluated against an invasive Helicobacter pylori strain. At four times the MIC, clarithromycin showed an early bactericidal effect within 4 h of incubation and, in determining the complete killing within a 16 h-incubation period, lansoprazole and rifabutin showed comparable activity, yielding bactericidal activities within 4 and 8 h of incubation, respectively. Amoxicillin and metronidazole showed bacteriostatic activity only.

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Figures

FIG. 1
FIG. 1
Number of H. pylori UdA203 organisms internalized by HEp-2 cells as determined by gentamicin survival assay.
FIG. 2
FIG. 2
Intracellular killing activity of amoxicillin (a), clarithromycin (b), lansoprazole (c), metronidazole (d), and rifabutin (e) against the H. pylori invasive strain UdA203. Bacterial counts were determined at time zero and after 2, 4, 8, 24, and 48 h of incubation with different antibiotic concentrations (⧫, control; ■, one times the MIC; ▴, two times the MIC; ×, four times the MIC). Mean values from triplicate measurements are shown. Bars indicate the standard deviations.
FIG. 2
FIG. 2
Intracellular killing activity of amoxicillin (a), clarithromycin (b), lansoprazole (c), metronidazole (d), and rifabutin (e) against the H. pylori invasive strain UdA203. Bacterial counts were determined at time zero and after 2, 4, 8, 24, and 48 h of incubation with different antibiotic concentrations (⧫, control; ■, one times the MIC; ▴, two times the MIC; ×, four times the MIC). Mean values from triplicate measurements are shown. Bars indicate the standard deviations.
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