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. 2000 Oct;79(4):2199-208.
doi: 10.1016/S0006-3495(00)76468-X.

The use of pHluorins for optical measurements of presynaptic activity

S Sankaranarayanan  1 , D De Angelis, J E Rothman, T A Ryan
Affiliations

The use of pHluorins for optical measurements of presynaptic activity

S Sankaranarayanan et al. Biophys J. 2000 Oct.

Abstract

Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.

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References

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