Skip to main page content
U.S. flag
See More

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Jun 15;18(12):3232-40.
doi: 10.1093/emboj/18.12.3232.

Constitutively active Pto induces a Prf-dependent hypersensitive response in the absence of avrPto

J P Rathjen  1 , J H Chang, B J Staskawicz, R W Michelmore
Affiliations

Constitutively active Pto induces a Prf-dependent hypersensitive response in the absence of avrPto

J P Rathjen et al. EMBO J. .

Abstract

Resistance in tomato to Pseudomonas syringae pv tomato (avrPto) is conferred by the gene Pto in a gene-for-gene relationship. A hypersensitive disease resistance response (HR) is elicited when Pto and avrPto are expressed experimentally within the same plant cell. The kinase capability of Pto was required for AvrPto-dependent HR induction. Systematic mutagenesis of the activation segment of Pto kinase confirmed the homologous P+1 loop as an AvrPto-binding determinant. Specific amino acid substitutions in this region led to constitutive induction of HR upon expression in the plant cell in the absence of AvrPto. Constitutively active Pto mutants required kinase capability for activity, and were unable to interact with proteins previously shown to bind to wild-type Pto. The constitutive gain-of-function phenotype was dependent on a functional Prf gene, demonstrating activation of the cognate disease resistance pathway and precluding a role for Prf upstream of Pto.

PubMed Disclaimer

References

    1. Science. 1994 Aug 12;265(5174):966-70 - PubMed
    1. Plant Cell. 1999 Jan;11(1):15-29 - PubMed
    1. Cell. 1996 Apr 19;85(2):149-58 - PubMed
    1. Cell. 1995 Dec 15;83(6):925-35 - PubMed
    1. FASEB J. 1995 Oct;9(13):1255-66 - PubMed

Publication types

MeSH terms