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Expression of miR-206 during the initiation of mammary gland development

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MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translational repression or RNA degradation. The aberrant expression of miRNAs might be involved in human diseases, including cancer. The expression of miR-206 in estrogen receptor alpha (ER-α)-positive human breast cancer tissues is well known. However, the expression and regulation of miR-206 in the developing mammary gland has not yet been studied. To understand the effects of miR-206 on mammary gland development, we have profiled gene expression in scramble-transfected and miR-206-overexpressing developing mammary buds. The genes that are potentially regulated by miR-206 in the mammary epithelium and/or mesenchyme, such as Tachykinin1 and Gata3, are known to be breast cancer markers. The expression of Wnt, which is involved in gland positioning, and of the transcription factors Tbx3 and Lef1, which are essential for mammary gland development, changes after miR-206 overexpression. Using a mammary bud in vitro culture system, we have demonstrated that miR-206 acts downstream of ER-α during mammary gland growth. Thus, miR-206 might be a novel candidate for morphogenesis during the initiation of mammary gland formation and the regulation of genes related to mammary gland development and breast cancer.

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Authors and Affiliations

  1. Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemoon-Gu, Seoul, 120-752, Korea

    Min-Jung Lee, Kyung-Sik Yoon, Kyoung-Won Cho & Han-Sung Jung

  2. Graduate School of Biomedical Science and Engineering, College of Medicine, Hanyang University, Seoul, Korea

    Kye-Seong Kim

  3. Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, Hong Kong

    Han-Sung Jung

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  1. Min-Jung Lee
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  2. Kyung-Sik Yoon
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  3. Kyoung-Won Cho
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  4. Kye-Seong Kim
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Corresponding author

Correspondence to Han-Sung Jung.

Additional information

This work was supported by a grant for the Bio and Medical Technology Development Program (2012M3A9B4028738) funded by the National Research Foundation of Korea (NRF) of the Ministry of Education, Science and Technology (MEST), Republic of Korea.

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Lee, MJ., Yoon, KS., Cho, KW. et al. Expression of miR-206 during the initiation of mammary gland development. Cell Tissue Res 353, 425–433 (2013). https://doi.org/10.1007/s00441-013-1653-3

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