Summary
In a survey of 35 strains ofStreptococcus thermophilus, 13 strains were found to harbor plasmid DNA. Most of these strains contained plasmid species varying in size from 2.2 to 7.15 kilobases. Only three strains had more than one plasmid species. Each of the nine distinct types of plasmid DNAs identified had two or more unique recognition sites for restriction endonucleases. The characteristics of the indigenous cryptic plasmids ofS. thermophilus may allow their development as cloning vectors useful in the genetic engineering of this species and other streptococci that are important in food production
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References
Auclair, J. and J.P. Accolas. 1983. Use of thermophilic lactic startes in the dairy industry. Antonie van Leeuwenhoek 49: 313–326.
Anderson, D.G. and L.L. McKay. 1984. In vivo cloning oflac genes inStreptococcus lactis ML3. Appl. Environ. Microbiol. 47: 245–249.
Behnke, D. 1981. Plasmid transformation ofStreptococcus sanguis (Challis) occurs by circular and linear molecules. Mol. Gen. Genet. 182: 490–497.
Chassy, B.M. 1976. A gentle method for the lysis of oral streptococci. Biochem. Biophys. Res. Commun. 68: 603–608.
Chopin, M.C., A. Moillo-Batt and A. Rouault. 1985. Plasmid-mediated UV-protection inStreptococcus lactis. FEMS Microbiol. Lett. 26: 243–245.
Davies, F.L. and M.J. Gasson. 1983. Genetics of dairy cultures. Ir. Food Sci. Technol. 7: 49–60.
Davies, F.L., H.M. Underwood and M.J. Gasson. 1981. The value of plasmid profiles for strain identification in lactic streptococci and the relationship betweenStreptococcus lactis 712, ML3 and C2. J. Appl. Bacteriol. 51: 325–337.
Gryczan, T.J. and D. Dubnau. 1978. Construction and properties of chimeric plasmids inBacillus subtilis. Proc. Natl. Acad. Sci. USA 75: 1428–1432.
Herman, R.E. and L.L. McKay. 1985. Isolation and partial characterization of plasmid DNA fromStreptococcus thermophilus. Appl. Environ. Microbiol. 50: 1103–1106.
Klaenhammer, T.R., L.L. McKay and K. Baldwin. 1978. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid. Appl. Environ. Microbiol. 35: 592–600.
Kondo, J.K. and L.L. McKay. 1985. Gene transfer systems and molecular cloning in group N streptococci: a review. J. Dairy Sci. 68: 2143–2159.
Macrina, F.L., D.J. Kopecko, K.R. Jones, D.J. Ayers and S.M. McCowen. 1978. A multiple plasmid containingEscherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1: 417–420.
Macrina, F.L., K.R. Jones and P.H. Wood. 1980. Chimeric streptococci plasmids and their use as molecular cloning vehicles inStreptococcus sanguis (Challis). J. Bacteriol. 143: 1425–1435.
McKay, L.L. 1983. Functional properties of plasmids in lactic streptococci. Antonie van Leeuwenhoek 49: 259–274.
O'Farrell, P.H., E. Kutter and M. Nakanishi. 1980. A restriction map of the bacteriophage T4 genome. Mol. Gen. Genet. 179: 421–435.
Oppenheim, A. 1981. Separation of closed circular DNA from linear DNA by electrophoresis in two dimensions in agarose gels. Nucleic Acids Res. 9: 6805–6812.
Pechman, H. and M. Teuber. 1980. Plasmid pattern of group N (lactic) streptococci. Zentralbl. Bacteriol. Abt. I, Orig. C. 1: 133–136.
Somkuti, G.A. and D.H. Steinberg. 1979. Adaptability ofStreptococcus thermophilus to lactose, glucose, and galactose. J. Food Protect. 42: 885–887.
Somkuti, G.A. and D.H. Steinberg. 1986. General method for plasmid DNA isolation from thermophilic lactic acid bacteria. J. Biotechnol., 3: 323–332.
Stafford, D.W. and D. Bieber. 1975. Concentration of DNA solutions by extraction with 2-butanol. Biochim. Biophys. Acta 378: 18–21.
Stougaard, P. and S. Molin. 1981. Vertical dye-bouyant density gradients for rapid analysis and preparation of plasmid DNA. Anal. Biochem. 118: 191–193.
Yang, R.C.-A., J. Lis and R. Wu. 1979. Elution of DNA from agarose gels after electrophoresis. Methods Enzymol. 68: 176–182.
Yu, R.S.T., T.V. Huang and A.A. Azad. 1983. Plasmid complement and sequence homology of some technological strains of lactic streptococci. Aust. J. Dairy Technol. 38: 104–109.
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Somkuti, G.A., Steinberg, D.H. Distribution and analysis of plasmids inStreptococcus thermophilus . Journal of Industrial Microbiology 1, 157–163 (1986). https://doi.org/10.1007/BF01569267
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DOI: https://doi.org/10.1007/BF01569267


