Skip to main content
Springer Nature Link
Log in

Diagnostic Oligonucleotide Microarrays for Microbiology

  • Chapter
A Beginner’s Guide to Microarrays

  • 424 Accesses

  • 12 Citations

Abstract

See More

This chapter deals with oligonucleotide microarrays for the detection and quantification of microorganisms.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

eBook
USD 23.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

') var buybox = document.querySelector("[data-id=id_"+ timestamp +"]").parentNode var buyingOptions = buybox.querySelectorAll(".buying-option") ;[].slice.call(buyingOptions).forEach(initCollapsibles) var buyboxMaxSingleColumnWidth = 480 function initCollapsibles(subscription, index) { var toggle = subscription.querySelector(".buying-option-price") subscription.classList.remove("expanded") var form = subscription.querySelector(".buying-option-form") var priceInfo = subscription.querySelector(".price-info") var buyingOption = toggle.parentElement if (toggle && form && priceInfo) { toggle.setAttribute("role", "button") toggle.setAttribute("tabindex", "0") toggle.addEventListener("click", function (event) { var expandedBuyingOptions = buybox.querySelectorAll(".buying-option.expanded") var buyboxWidth = buybox.offsetWidth ;[].slice.call(expandedBuyingOptions).forEach(function(option) { if (buyboxWidth <= buyboxMaxSingleColumnWidth && option != buyingOption) { hideBuyingOption(option) } }) var expanded = toggle.getAttribute("aria-expanded") === "true" || false toggle.setAttribute("aria-expanded", !expanded) form.hidden = expanded if (!expanded) { buyingOption.classList.add("expanded") } else { buyingOption.classList.remove("expanded") } priceInfo.hidden = expanded }, false) } } function hideBuyingOption(buyingOption) { var toggle = buyingOption.querySelector(".buying-option-price") var form = buyingOption.querySelector(".buying-option-form") var priceInfo = buyingOption.querySelector(".price-info") toggle.setAttribute("aria-expanded", false) form.hidden = true buyingOption.classList.remove("expanded") priceInfo.hidden = true } function initKeyControls() { document.addEventListener("keydown", function (event) { if (document.activeElement.classList.contains("buying-option-price") && (event.code === "Space" || event.code === "Enter")) { if (document.activeElement) { event.preventDefault() document.activeElement.click() } } }, false) } function initialStateOpen() { var buyboxWidth = buybox.offsetWidth ;[].slice.call(buybox.querySelectorAll(".buying-option")).forEach(function (option, index) { var toggle = option.querySelector(".buying-option-price") var form = option.querySelector(".buying-option-form") var priceInfo = option.querySelector(".price-info") if (buyboxWidth > buyboxMaxSingleColumnWidth) { toggle.click() } else { if (index === 0) { toggle.click() } else { toggle.setAttribute("aria-expanded", "false") form.hidden = "hidden" priceInfo.hidden = "hidden" } } }) } initialStateOpen() if (window.buyboxInitialised) return window.buyboxInitialised = true initKeyControls() })()

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  1. Alon U, Barkai N, Notterman DA, Gish K, Ybarra S, Mack D, Levine AJ (1999) Broad patterns of gene expression revealed by clustering analysis of tumor and normal colon tissues probed by oligonucleotide arrays. Proc Natl Acad Sci USA 96: 6745–6750.

    Article  PubMed  CAS  Google Scholar 

  2. Amann RI, Ludwig W, Schleifer KH (1995) Phylogenic Identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59: 143–169.

    PubMed  CAS  Google Scholar 

  3. Amann R, Ludwig W (2000) Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology. FEMS Microbiol Rev 24: 555–565.

    Article  PubMed  CAS  Google Scholar 

  4. Anthony RM, Brown TJ, French GL (2000) Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. J Clin Microbiol 38: 781–788.

    PubMed  CAS  Google Scholar 

  5. Auman AJ, Stolyar S, Costello AM, Lidstrom ME (2000) Molecular characterization of methanotrophic isolates from freshwater lake sediment. Appl Environ Microbiol 66: 5259–5266.

    Article  PubMed  CAS  Google Scholar 

  6. Bavykin SG, Akowski JP, Zakhariev VM, Barsky VE, Perov AN, Mirzabekov AD (2001) Portable system for microbial sample preparation and oligonucleotide microarray analysis. Appl Environ Microbiol 67: 922–928.

    Article  PubMed  CAS  Google Scholar 

  7. Behr T, Koob C, Schedl M, Mehlen A, Meier H, Knopp D, Frahm E, Obst U, Schleifer K, Niessner R, Ludwig W (2000) A nested array of rRNA targeted probes for the detection and identification of enterococci by reverse hybridization. Syst Appl Microbiol 23: 563–572.

    Article  PubMed  CAS  Google Scholar 

  8. Bourne DG, McDonald IR, Murrell JC (2001) Comparison of pmoA PCR primer sets as tools for investigating methanotroph diversity in three Danish soils. Appl Environ Microbiol 67: 3802–3809.

    Article  PubMed  CAS  Google Scholar 

  9. Breslauer KJ, Frank R, Blocker H, Marky LA (1986) Predicting DNA duplex stability from the base sequence. Proc Natl Acad Sci U S A 83: 3746–3750.

    Article  PubMed  CAS  Google Scholar 

  10. Call DR, Chandler DP, Brockman F (2001) Fabrication of DNA microarrays using unmodified oligonucleotide probes. Biotechniques 30: 368–72, 374, 376.

    PubMed  CAS  Google Scholar 

  11. Chizhikov V, Rasooly A, Chumakov K, Levy DD (2001) Microarray analysis of microbial virulence factors. Appl Environ Microbiol 67: 3258–3263.

    Article  PubMed  CAS  Google Scholar 

  12. Chizhikov, V., M. Wagner, A. Ivshina, Y. Hoshino, A. Z. Kapikian, and K. Chumakov. 2002. Detection and Genotyping of Human Group A Rotaviruses by Oligonucleotide Microarray Hybridization. J. Clin. Microbiol 40:2398–2407.

    Google Scholar 

  13. Cho JC, Tiedje JM (2001) Bacterial species determination from dna-dna hybridization by using genome fragments and dna microarrays. Appl Environ Microbiol 67: 3677–3682.

    Article  PubMed  CAS  Google Scholar 

  14. Cho JC, Tiedje JM (2002) Quantitative d etection of microbial genes by using DNA microarrays. Appl Environ Microbiol 68: 1425–1430.

    Article  PubMed  CAS  Google Scholar 

  15. Connors TD, Burn TC, VanRaay T, Germino GG, Klinger KW, Landes GM (1997) Evaluation of DNA sequencing ambiguities using tetramethylammonium chloride hybridization conditions. Biotechniques 22: 1088–1090.

    PubMed  CAS  Google Scholar 

  16. Dahllof I, Baillie H, Kjelleberg S (2000) rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity. Appl Environ Microbiol 66:3376–3380.

    Article  PubMed  CAS  Google Scholar 

  17. de Saizieu A, Gardes C, Flint N, Wagner C, Kamber M, Mitchell TJ, Keck W, Amrein KE, Lange R (2000) Microarray-based identification of a novel Streptococcus pneumoniae regulon controlled by an autoinduced peptide. J Bacteriol 182: 4696–4703.

    Article  PubMed  Google Scholar 

  18. Edgcomb, V. P., D. T. Kysela, A. Teske, G A. de Vera, and M. L. Sogin. 2002. Benthic eukaryotic diversity i n the Guaymas Basin hydrothermal vent environment. Proc. Natl. Acad. Sci. U. S. A 99:7658–7662.

    Google Scholar 

  19. Edwards, U., T. Rogall, H. Blocker, M. Emde, and E. C. Bottger. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 17:7843–7853.

    Google Scholar 

  20. Erdogan F, Kirchner R, Mann W, Ropers HH, Nuber UA (2001) Detection of mitochondrial single nucleotide polymorphisms using a primer elongation reaction on oligonucleotide microarrays. Nucleic Acids Res 29: E36.

    Article  PubMed  CAS  Google Scholar 

  21. Fotin AV, Drobyshev AL, Proudnikov DY, Perov AN, Mirzabekov AD (1998) Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips. Nucleic Acids Res 26: 1515–1521.

    Article  PubMed  CAS  Google Scholar 

  22. Giovannoni, S. J. 1991. The polymerase chain reaction, p. 177–203. In E. Stackebrandt and M. Goodfellow (eds.), Nucleic acid techniques in bacterial systematics. Wiley and Sons., Chichester.

    Google Scholar 

  23. Gruber HJ, Hahn CD, Kada G, Riener CK, Harms GS, Ahrer W, Dax TG, Knaus HG (2000) Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Bioconjug Chem 11:696–704.

    Article  PubMed  CAS  Google Scholar 

  24. Gunderson KL, Huang XC, Morris MS, Lipshutz RJ, Lockhart DJ, Chee MS (1998) Mutation detection by ligation to complete n-mer DNA arrays. Genome Res 8: 1142–1153.

    PubMed  CAS  Google Scholar 

  25. Guo Z, Guilfoyle RA, Thiel AJ, Wang R, Smith LM (1994) Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Res 22: 5456–5465.

    Article  PubMed  CAS  Google Scholar 

  26. Guschin DY, Mobarry BK, Proudnikov D, Stahl DA, Rittmann BE, Mirzabekov AD (1997) Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology. Appl Environ Microbiol 63: 2397–2402.

    PubMed  CAS  Google Scholar 

  27. Hacia JG (1999) Resequencing and mutational analysis using oligonucleotide microarrays. Nat Genet 21: 42–47.

    Article  PubMed  CAS  Google Scholar 

  28. Hamels S, Gala JL, Dufour S, Vannuffel P, Zammatteo N, Remacle J (2001) Consensus PCR and microarray for diagnosis of the genus Staphylococcus, species, and methicillin resistance. Biotechniques 31: 1364–2.

    PubMed  CAS  Google Scholar 

  29. Hansen KH, Ahring BK, Raskin L (1999) Quantification of syntrophic fatty acid-betaoxidizing bacteria in a mesophilic biogas reactor by oligonucleotide probe hybridization. Appl Environ Microbiol 65: 4767–4774.

    PubMed  CAS  Google Scholar 

  30. Hershberger, K. L., S. M. Barns, A. L. Reysenbach, S. C. Dawson, and N. R. Pace. 1996. Wide diversity of Crenarchaeota. Nature 384:420.

    Google Scholar 

  31. Hung, S. H., Yu, Q., Gray, D. M., and Ratliff, R. L. Evidence from CD spectra that d(purine).r(pyrimidine) and r(purine).d(pyrimidine) hybrids are in different structural classes. Nucleic Acids Res. 22[20], 4326–4334. 11-10-1994.

    Google Scholar 

  32. Ishii K, Fukui M (2001) Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate pcr. Appl Environ Microbiol 67: 3753–3755.

    Article  PubMed  CAS  Google Scholar 

  33. Koizumi, Y., J. J. Kelly, T. Nakagawa, H. Urakawa, S. El Fantroussi, S. Al Muzaini, M. Fukui, Y Urushigawa, and D. A. Stahl. 2002. Parallel characterization of anaerobic toluene-and ethylbenzene-degrading microbial consortia by PCR-denaturing gradient gel electrophoresis, RNA-DNA membrane hybridization and DNA microarray technology. Appl Environ Microbiol 68:3215–3225.

    Google Scholar 

  34. Kolganova, T. V., B. B. Kuznetsov, and T. P. Turova. 2002. Designing and testing oligonucleotide primers for amplification and sequencing of archaeal 16S rRNA genes. Mikrobiologiia 71:283–286.

    Google Scholar 

  35. Lapa, S., M. Mikheev, S. Shchelkunov, V. Mikhailovich, A. Sobolev, V. Blinov, I. Babkin, A. Guskov, E. Sokunova, A. Zasedatelev, L. Sandakhchiev, and A. Mirzabekov. 2002. Species-level identification of orthopoxviruses with an oligonucleotide microchip. J. Clin. Microbiol 40:753–757.

    Google Scholar 

  36. Lindroos K, Liljedahl U, Raitio M, Syvanen AC (2001) Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries. Nucleic Acids Res 29: E69–E69.

    Article  PubMed  CAS  Google Scholar 

  37. Lipshutz RJ, Fodor SP, Gingeras TR, Lockhart DJ (1999) High density synthetic oligonucleotide arrays. Nat Genet 21: 20–24.

    Article  PubMed  CAS  Google Scholar 

  38. Lovell CR, Friez MJ, Longshore JW, Bagwell CE (2001) Recovery and phylogenetic analysis of nifH sequences from diazotrophic bacteria associated with dead aboveground biomass of Spartina alterniflora. Appl Environ Microbiol 67: 5308–5314.

    Article  PubMed  CAS  Google Scholar 

  39. Lueders T, Chin KJ, Conrad R, Friedrich M (2001) Molecular analyses of methyl-coenzyme M reductase alpha-subunit (mcrA) genes in rice field soil and enrichment cultures reveal the methanogenic phenotype of a novel archaeal lineage. Environ Microbiol 3: 194–204.

    Article  PubMed  CAS  Google Scholar 

  40. Maidak BL, Larsen N, McCaughey MJ, Overbeek R, Olsen GJ, Fogel K, Blandy J, Woese CR (1994) The ribosomal database project. Nucl Acid Res 22: 3485–3487.

    Article  CAS  Google Scholar 

  41. Majlessi, M., N. C. Nelson, and M. M. Becker. 1998. Advantages o f 2′-O-methyl oligoribonucleotide probes for detecting RNA targets. Nucleic Acids Res. 26:2224–2229.

    Google Scholar 

  42. Martin-Laurent, F., L. Philippot, S. Hallet, R. Chaussod, J. C. Germon, G Soulas, and G Catroux. 2001. Dna extraction from soils: old bias for new microbial diversity analysis methods. Appl. Environ. Microbiol. 67:2354–2359.

    Google Scholar 

  43. Matsunaga, T., H. Nakayama, M. Okochi, and H. Takeyama. 2002. Fluorescent detection of cyanobacterial DNA using bacterial magnetic particles on a MAG-microarray. Biotechnol. Bioeng. 73:400–405.

    Google Scholar 

  44. McDonald IR, Murrell JC (1997) The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs. Appl Environ Microbiol 63:3218–3224.

    PubMed  CAS  Google Scholar 

  45. Mikhailovich, V., S. Lapa, D. Gryadunov, A. Sobolev, B. Strizhkov, N. Chernyh, O. Skotnikova, O. Irtuganova, A. Moroz, V. Litvinov, M. Vladimirskii, M. Perelman, L. Chernousova, V. Erokhin, A. Zasedatelev, and A. Mirzabekov. 2001. Identification of rifampicin-resistant Mycobacterium tuberculosis strains by hybridization, PCR, and ligase detection reaction on oligonucleotide microchips. J._Clin. Microbiol 39:2531–2540.

    Google Scholar 

  46. Milcic-Terzic J, Lopez-Vidal Y, Vrvic MM, Saval S (2001) Detection of catabolic genes in indigenous microbial consortia isolated from adiesel-contaminated soil. Bioresources Technol 78: 47–54.

    Article  CAS  Google Scholar 

  47. Mir KU, Southern EM (1999) Determining the influence of structure on hybridization using oligonucleotide arrays. Nat Biotechnol 17: 788–792.

    Article  PubMed  CAS  Google Scholar 

  48. Murray AE, Lies D, Li G, Nealson K, Zhou J, Tiedje JM (2001) DNA/DNA hybridization to microarrays reveals gene-specific differences between closely related microbial genomes. Proc Natl Acad Sci U S A 98: 9853–9858.

    Article  PubMed  CAS  Google Scholar 

  49. Petersen, M., K. Bondensgaard, J. Wengel, and J. P. Jacobsen. 2002. Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA:RNA hybrids. J. Am Chem. Soc. 124:5974–5982.

    Google Scholar 

  50. Polz FM, Cavanaugh CM (1998) Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol 64: 3724–3730.

    PubMed  CAS  Google Scholar 

  51. Radajewski S, Ineson P, Parekh NR, Murrell JC (2000) Stable-isotope probing as a tool in microbial ecology. Nature 403:646–649.

    Article  PubMed  CAS  Google Scholar 

  52. Rainey FA, Ward-Rainey NL, Janssen PH, Hippe H, Stackebrandt E (1996) Clostridium paradoxum DSM 7308T contains multiple 16S rRNA genes with heterogeneous intervening sequences. Microbiology 142(Pt 8): 2087–2095.

    Article  PubMed  CAS  Google Scholar 

  53. Ramdas L, Coombes KR, Baggerly K, Abruzzo L, Highsmith WE, Krogmann T, Hamilton SR, Zhang W (2001) Sources of nonlinearity in cDNA microarray expression measurements. Genome Biol 2: RESEARCH0047.

    Article  PubMed  CAS  Google Scholar 

  54. Ren T, Roy R, Knowles R (2000) Production and consumption of nitric oxide by three methanotrophic bacteria. Appl Environ Microbiol 66: 3891–3897.

    Article  PubMed  CAS  Google Scholar 

  55. Reysenbachal, Giver LJ, Wickham GS, Pace NR (1992) Differential amplification of rRNA genes by polymerase chain reaction. Appl Environ Microbiol 58: 3417–3418.

    Google Scholar 

  56. Rudi K, Flateland SL, Hanssen JF, Bengtsson G, Nissen H (2002) Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere. Appl Environ Microbiol 68: 1146–1156.

    Article  PubMed  CAS  Google Scholar 

  57. Rudi K, Skulberg OM, Skulberg R, Jakobsen KS (2000) Application of sequence-specific labeled 16S rRNA gene oligonucleotide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization [In Process Citation]. Appl Environ Microbiol 66:4004–4011.

    Article  PubMed  CAS  Google Scholar 

  58. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor.

    Google Scholar 

  59. SantaLucia J, Jr., Allawi HT, Seneviratne PA (1996) Improved nearest-neighbor parameters for predicting DNA duplex stability. Biochemistry 35: 3555–3562.

    Article  PubMed  CAS  Google Scholar 

  60. Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray [see comments]. Science 270: 467–470.

    Article  PubMed  CAS  Google Scholar 

  61. Shchepinov MS, Case-Green SC, Southern EM (1997) Steric factors influencing hybridisation of nucleic acids to oligonucleotide arrays. Nucleic Acids Res 25: 1155–1161.

    Article  PubMed  CAS  Google Scholar 

  62. Small J, Call DR, Brockman FJ, Straub TM, Chandler DP (2001) Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays. Appl Environ Microbiol 67: 4708–4716.

    Article  PubMed  CAS  Google Scholar 

  63. Sohail M, Akhtar S, Southern EM (1999) The folding of large RNAs studied by hybridization to arrays of complementary oligonucleotides. RNA 5: 646–655.

    Article  PubMed  CAS  Google Scholar 

  64. Sohail M, Hochegger H, Klotzbucher A, Guellec RL, Hunt T, Southern EM (2001) Antisense oligonucleotides selected by hybridisation to scanning arrays are effective reagents in vivo. Nucleic Acids Res 29:2041–2051.

    Article  PubMed  CAS  Google Scholar 

  65. Southern EM (1996) DNA chips: analysing sequence by hybridization to oligonucleotides on a large scale. Trends Genet 12: 110–115.

    Article  PubMed  CAS  Google Scholar 

  66. Spiro A, Lowe M, Brown D (2000) A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry. Appl Environ Microbiol 66: 4258–4265.

    Article  PubMed  CAS  Google Scholar 

  67. Sugimoto N, Nakano M, Nakano S (2000) Thermodynamics-structure relationship of single mismatches in RNA/DNA duplexes. Biochemistry 39: 11270–11281.

    Article  PubMed  CAS  Google Scholar 

  68. Strunk, O., Gross, O., Reichel, B., May, M., Hermann, S., Stuckman, N., Nonhoff, B., Lenke, M., Ginhart, A., Vilbig, A., Ludwig, T, Bode, A., Schleifer, K.-H., and Ludwig, W. ARB: A software environment for for sequence data. 2000. www.mikro.biologie.tu-muenchen.de.

  69. Urakawa, H., P. A. Noble, S. El Fantroussi, J. J. Kelly, and D. A. Stahl. 2002. Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses. Appl. Environ. Microbiol. 68:235–244.

    Google Scholar 

  70. Voordouw G, Voordouw JK, Karkhoff-Schweizer RR, Fedorak PM, Westlake DWS (1991) Reverse sample genome probing, a new technique for identification of bacteria in environmental samples by DNA hybridization and its application to the identification of sulfate-reducing bacteria in oil field samples. Appl Environ Microbiol 57: 3070–3078.

    PubMed  CAS  Google Scholar 

  71. Westin L, Miller C, Vollmer D, Canter D, Radtkey R, Nerenberg M, O’Connell JP (2001) Antimicrobial resistance and bacterial identification utilizing a microelectronic chip array. J Clin Microbiol 39: 1097–1104.

    Article  PubMed  CAS  Google Scholar 

  72. Widmer F, Shaffer BT, Porteous LA, Seidler RJ (1999) Analysis of nifH gene pool complexity in soil and litter at a Douglas fir forest site in the Oregon cascade mountain range. Appl Environ Microbiol 65: 374–380.

    PubMed  CAS  Google Scholar 

  73. Wilson, K. H., W. J. Wilson, J. L. Radosevich, T. Z. DeSantis, V. S. Viswanathan, T. A. Kuczmarski, and G L. Andersen. 2002. High-density microarray of small-subunit ribosomal DNA probes. Appl Environ Microbiol 68:2535–2541.

    Google Scholar 

  74. Woese CR (1987) Bacterial evolution. Microbiol Rev 51: 221–271.

    PubMed  CAS  Google Scholar 

  75. Wood WI, Gitschier J, Lasky LA, Lawn RM (1985) Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci U S A 82: 1585–1588.

    Article  PubMed  CAS  Google Scholar 

  76. Wyman M, Davies JT, Crawford DW, Purdie D A (2000) M olecular and physiological responses of two classes of marine chromophytic phytoplankton (Diatoms and prymnesiophytes) during the development of nutrient-stimulated blooms. Appl Environ Microbiol 66: 2349–2357.

    Article  PubMed  CAS  Google Scholar 

  77. Yershov G, Barsky V, Belgovskiy A, Kirillov E, Kreindlin E, Ivanov I, Parinov S, Guschin D, Drobishev A, Dubiley S, Mirzabekov A (1996) DNA analysis and diagnostics on oligonucleotide microchips. Proc Natl Acad Sci U S A 93: 4913–4918.

    Article  PubMed  CAS  Google Scholar 

  78. Zheng D, Alm EW, Stahl DA, Raskin L (1996) Characterization of universal small-subunit rRNA h ybridization probes for quantitative molecular microbial ecology studies. Appl Environ Microbiol 62:4504–4513.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Austrian Research Centers, Seibersdorf, Austria

    Levente Bodrossy Ph.D.

Authors
  1. Levente Bodrossy Ph.D.
    View author publications

    Search author on:PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Kentucky Medical Center, USA

    Eric M. Blalock

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media New York

About this chapter

Cite this chapter

Bodrossy, L. (2003). Diagnostic Oligonucleotide Microarrays for Microbiology. In: Blalock, E.M. (eds) A Beginner’s Guide to Microarrays. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8760-0_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4419-8760-0_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4684-5

  • Online ISBN: 978-1-4419-8760-0

  • eBook Packages: Springer Book Archive

Keywords

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Publish with us

Policies and ethics